Kim Jae Yeon, Choi Jong Ho, Kim Se Ho, Park Hyeri, Lee Dongsook, Kim Gi Jin
Department of Biomedical Science, CHA University, Seongnam, Korea.
Department of Oral Pathology, College of Dentistry, Gangneung-Wonju National University, Gangneung, Korea.
Int J Stem Cells. 2021 Feb 28;14(1):112-118. doi: 10.15283/ijsc20117.
Mesenchymal stem cell (MSC)-based therapy using gene delivery systems has been suggested for degenerative diseases. Although MSC-based clinical applications are effective and safe, the mode of action remains unclear. Researchers have commonly applied viral-based gene modification because this system has efficient vehicles. While viral transfection carries many risks, such as oncogenes and chromosomal integration, nonviral gene delivery techniques are less expensive, easier to handle, and safe, although they are less efficient. The electroporation method, which uses Nucleofection technology, provides critical opportunities for hard-to-transfect primary cell lines, including MSCs. Therefore, to improve the therapeutic efficacy using genetically modified MSCs, researchers must determine the optimal conditions for the introduction of the Nucleofection technique in MSCs. Here, we suggest optimal methods for gene modification in PD-MSCs using an electroporation gene delivery system for clinical application.
基于间充质干细胞（MSC）并使用基因递送系统的疗法已被推荐用于治疗退行性疾病。尽管基于MSC的临床应用是有效且安全的，但其作用方式仍不清楚。研究人员通常采用基于病毒的基因修饰，因为该系统具有高效的载体。虽然病毒转染存在许多风险，如致癌基因和染色体整合，但非病毒基因递送技术成本较低、操作更简便且安全，尽管其效率较低。使用核转染技术的电穿孔方法为包括MSC在内的难以转染的原代细胞系提供了关键机遇。因此，为了提高使用基因修饰的MSC的治疗效果，研究人员必须确定在MSC中引入核转染技术的最佳条件。在此，我们提出了使用电穿孔基因递送系统对牙周膜来源的间充质干细胞（PD-MSCs）进行基因修饰的最佳方法，以供临床应用。
