Wang Meng-Qiu, Wang Chen-Xiao, Li Si, Zhao Wei, Ma Fang, Ma Xue
Department of Pediatric Urology, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China.
West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China.
Zhonghua Nan Ke Xue. 2020 Aug;26(8):681-685.
To investigate the effects of the enzyme activity of neuraminidase 1 (Neu1) on the biological behavior of prostate cancer PC3 and DU145 cell lines.
We detected the expression of Neul in the prostate cancer PC3 and DU145 cell lines by Western blot. Using sialidase inhibitors and antibody blocking, we suppressed the enzyme activity of Neu1 and then measured the proliferation and invasiveness of the two cell lines by CCK-8 and Transwell assay, respectively.
No statistically significant difference was found in the Neu1 expression between the PC3 and DU145 cell lines. The proliferation and invasiveness of the two types of cells were both increased after inhibition of the Neu1 enzyme activity.
The enzyme activity of Neu1 is correlated with the biological behavior of prostate cancer PC3 and DU145 cells and capable of inhibiting the proliferation and invasiveness of the two types of cells.
研究神经氨酸酶1（Neu1）的酶活性对前列腺癌PC3和DU145细胞系生物学行为的影响。
通过蛋白质免疫印迹法检测Neu1在前列腺癌PC3和DU145细胞系中的表达。使用唾液酸酶抑制剂和抗体阻断，抑制Neu1的酶活性，然后分别通过CCK-8和Transwell实验检测这两种细胞系的增殖和侵袭能力。
PC3和DU145细胞系之间的Neu1表达无统计学显著差异。抑制Neu1酶活性后，两种类型细胞的增殖和侵袭能力均增强。
Neu1的酶活性与前列腺癌PC3和DU145细胞的生物学行为相关，并且能够抑制这两种细胞的增殖和侵袭能力。
