Department of Clinical Microbiology, Faculty of Medicine, Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta.
Acta Med Indones. 2020 Oct;52(4):352-359.
Indonesia is one of the countries with the highest incidence of tuberculosis (TB) in the world. Appropriate diagnosis is an effort to control TB. The World Health Organization has recommended loop-mediated isothermal amplification (LAMP). In order to be applied routinely, it is necessary to do research to evaluate the LAMP method.
the research was a cross-sectional study and was carried out at the Clinical Microbiology Laboratory of the Faculty of Medicine, Universitas Indonesia, for 100 patients suspected of having pulmonary TB. Each patient handed over two direct sputum specimens. For each direct sputum specimen, an acid-fast bacilli (AFB) smear was carried out. Two direct sputum specimens from each patient were combined to produce mixed sputum. For each mixed sputum specimen, an AFB smear, Lowenstein-Jensen culture, and TB-LAMP were carried out.
the percentage of LAMP (+) cells in mixed sputum that was AFB (-) was 32.78%. The TB-LAMP showed a sensitivity of 100% (95% CI 89.56-100%), a specificity of 69.64% (95% CI 55.74-80.84%), positive predictive value of 71.19% (95% CI 57.73-81.86%), and negative predictive value of 100% (95% CI 88.83-100%).
TB-LAMP has both high sensitivity and negative predictive value.
印度尼西亚是世界上结核病（TB）发病率最高的国家之一。适当的诊断是控制结核病的一种努力。世界卫生组织（WHO）推荐环介导等温扩增（LAMP）。为了能够常规应用，有必要进行研究来评估 LAMP 方法。
该研究为横断面研究，在印度尼西亚大学医学院临床微生物学实验室进行，共纳入 100 例疑似患有肺结核的患者。每位患者提供两份直接痰液标本。每份直接痰液标本均进行抗酸杆菌（AFB）涂片检查。每位患者的两份直接痰液标本混合后产生混合痰液。每份混合痰液标本均进行 AFB 涂片、Lowenstein-Jensen 培养和 TB-LAMP 检查。
混合痰液中 AFB（-）的 LAMP（+）细胞百分比为 32.78%。TB-LAMP 的灵敏度为 100%（95%CI 89.56-100%），特异性为 69.64%（95%CI 55.74-80.84%），阳性预测值为 71.19%（95%CI 57.73-81.86%），阴性预测值为 100%（95%CI 88.83-100%）。
TB-LAMP 具有较高的灵敏度和阴性预测值。
