The effect of phenylglyoxal on the translocation of pyruvate in rat-heart mitochondria.

The effect of phenylglyoxal, an arginine-specific reagent, on the translocation of pyruvate and on the binding of alpha-cyanocinnamate by rat-heart mitochondria has been studied. It has been found that both the uptake and the oxidation of pyruvate by mitochondria are inhibited by phenylglyoxal. The inhibitory potency increases with the increasing of the pH of the medium. Phenylglyoxal does not affect the transmembrane delta pH. Phenylglyoxal also inhibits the binding of alpha-cyanocinnamate to mitochondria. Substrates of the carrier, such as pyruvate itself and monochloroacetate, partially prevent the inhibition of alpha-cyanocinnamate binding by phenylglyoxal, whilst acetate has no effect in this respect. Phenylglyoxal affects only the affinity of the alpha-cyanocinnamate binding site(s), without changing their total number. The results obtained indicate that arginine residues are involved in the mechanism of pyruvate translocation and of alpha-cyanocinnamate binding in rat-heart mitochondria.