Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Viña del Mar, Chile.
Adv Exp Med Biol. 2021;1326:55-72. doi: 10.1007/5584_2020_607.
Lin28 is a highly conserved RNA binding protein that regulates stemness whose molecular role has been widely studied in vitro. However, the regulation and the molecular role of Lin28 during the development of the vertebrate central nervous system (CNS) in vivo are not completely understood. Here, the expression and the putative role of Lin28 in the development of the mammalian CNS are reviewed in the context of recent results showing the progressive cellular and molecular changes in neural progenitor cells. Downstream genes that may play a role during CNS development and the effect of misregulated expression of Lin28 are discussed. Evidence suggests that Lin28 promotes symmetric divisions over asymmetric divisions, increasing the number of progenitors during early neurogenesis. Future quantitative analysis of Lin28 isoforms levels and stabilities together with single cell transcriptomics data, cell cycle dynamics and cell fate analysis in Lin28 gain- and loss-of-function experiments will provide a better understanding of the molecular role of Lin28 during development.
Lin28 是一种高度保守的 RNA 结合蛋白，可调节干性，其分子作用在体外已得到广泛研究。然而，Lin28 在脊椎动物中枢神经系统（CNS）发育过程中的调节和分子作用尚不完全清楚。本文在最近显示神经祖细胞中细胞和分子变化的结果的背景下，综述了 Lin28 在哺乳动物 CNS 发育中的表达和可能作用。讨论了可能在 CNS 发育过程中发挥作用的下游基因以及 Lin28 表达失调的影响。有证据表明，Lin28 促进对称分裂而不是不对称分裂，从而在早期神经发生过程中增加祖细胞的数量。Lin28 功能获得和功能丧失实验中 Lin28 同工型水平和稳定性的定量分析以及单细胞转录组学数据、细胞周期动力学和细胞命运分析将为 Lin28 在发育过程中的分子作用提供更好的理解。
