Hospital of Chengdu, University of Traditional Chinese Medicine, Chengdu, China.
Chengdu University of Traditional Chinese Medicine Clinical Medical College, Chengdu, China.
PLoS One. 2020 Dec 30;15(12):e0243987. doi: 10.1371/journal.pone.0243987. eCollection 2020.
Diabetic macroangiopathy is part of the most common serious complications of diabetes. Previous studies indicate that lncRNAs involved in the process of diabetes and another vascular disease. However, their detailed mechanism of the lncRNAs involved in diabetic macroangiopathy has not been well characterized. In the present study, we generated rat models of diabetic macroangiopathy induced by High fat of 16weeks. A total of 15 GK rats were constructed as a test group, along with 15 Wistar rats set as control group, and thoracic aorta tissue from each group was collected. Whole genomic RNA sequencing was performed on thoracic aorta tissue; 3223 novel lncRNAs and 20367 annotated lncRNAs were indemnified in thoracic aorta samples, and 864 lncRNAs were expressed differently in the test and control groups. Gene ontology term enrichment showed the apparent enrichment of inflammatory response and cell apoptosis, which consistent with the results of H&E Staining, TUNEL Assay, and ELISA; Extensive literature reveals inflammatory response and cell apoptosis play an important role in the process of diabetic macroangiopathy. The results of the present study indicated that lncRNAs, especially Nrep. bSep08, Col5a1, aSep0, soygee.aSep08-unspliced, NONRATT013247.2, votar.aSep08-unspliced, etc, both participate in and mediate the process of inflammatory response, cell apoptosis. What's more. Our research provides further insights into understanding of the basic molecular mechanisms underlying diabetic macroangiopathy.
糖尿病大血管病变是糖尿病最常见的严重并发症之一。先前的研究表明，长链非编码 RNA 参与了糖尿病和另一种血管疾病的发生过程。然而，其在糖尿病大血管病变中的详细作用机制尚未得到很好的描述。在本研究中，我们构建了 16 周高脂诱导的糖尿病大血管病变大鼠模型。共构建 15 只 GK 大鼠作为实验组，同时构建 15 只 Wistar 大鼠作为对照组，并收集每组的胸主动脉组织。对胸主动脉组织进行全基因组 RNA 测序；在胸主动脉样本中鉴定出 3223 个新的长链非编码 RNA 和 20367 个注释长链非编码 RNA，864 个长链非编码 RNA在实验组和对照组之间表达差异。基因本体论术语富集显示炎症反应和细胞凋亡的明显富集，与 H&E 染色、TUNEL 检测和 ELISA 的结果一致；广泛的文献表明炎症反应和细胞凋亡在糖尿病大血管病变的过程中起着重要作用。本研究结果表明，长链非编码 RNA，特别是 Nrep.bSep08、Col5a1、aSep0、soygee.aSep08-unspliced、NONRATT013247.2、votal.aSep08-unspliced 等，既参与又介导炎症反应、细胞凋亡的过程。更重要的是，我们的研究为理解糖尿病大血管病变的基本分子机制提供了进一步的认识。
