Bruni R, Baritussio A, Quaglino D, Gabelli C, Benevento M, Ronchetti I P
Istituto di Medicina Interna, Università di Padova, Italy.
Biochim Biophys Acta. 1988 Feb 4;958(2):255-67. doi: 10.1016/0005-2760(88)90184-1.
To clarify perinatal transformations of surfactant we performed lung lavage in term fetuses and in 0-24-h-old newborn rabbits. Lavage fluid was separated into three pools, namely lavage pellet, lavage supernatant and cells. We found that at birth the pellet contains 94.1 +/- 1.4% (S.E.) saturated phosphatidylcholine, while the supernatant and cells contain traces of it. At birth the pellet contains secreted lamellar bodies while the supernatant lacks any recognizable structure. After birth, the alveolar saturated phosphatidylcholine level increases 5.1-times in 24 h, the proportions between pools reaching adult values in 90 min (pellet = 75.9 + 4.8%, supernatant = 22.7 +/- 4.9%), and small vesicles appear in the supernatant, probably originating from the turnover of alveolar surfactant during breathing. The saturated phosphatidylcholine associated with cells remains unchanged. At birth, the 32-38 kDa surfactant apolipoprotein appears to be less extensively sialylated than in adult life.
为阐明表面活性剂的围产期转变，我们对足月胎儿和出生0 - 24小时的新生兔进行了肺灌洗。灌洗液被分为三个部分，即灌洗沉淀、灌洗上清液和细胞。我们发现，出生时沉淀中含有94.1±1.4%（标准误）的饱和磷脂酰胆碱，而上清液和细胞中仅含微量。出生时沉淀中含有分泌的板层小体，而上清液中没有任何可识别的结构。出生后，肺泡饱和磷脂酰胆碱水平在24小时内增加5.1倍，各部分之间的比例在90分钟时达到成人水平（沉淀 = 75.9 + 4.8%，上清液 = 22.7±4.9%），上清液中出现小泡，可能源于呼吸过程中肺泡表面活性剂的周转。与细胞相关的饱和磷脂酰胆碱保持不变。出生时，32 - 38 kDa的表面活性剂载脂蛋白的唾液酸化程度似乎低于成年期。
