State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, Research Center for Traceability and Standardization of TCMs, Cellular and Molecular Biology Center. School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, P.R. China.
Shenzhen Institute for Drug Control, Shenzhen, P.R. China.
J Sep Sci. 2021 Mar;44(5):1062-1071. doi: 10.1002/jssc.202001087. Epub 2021 Jan 26.
Angelicae Sinensis Radix is a world-renowned herbal medicine originating in China. Owing to many environmental and geographical factors, Angelicae Sinensis Radix from various origins may have a difference in the content of ingredients, which made the confusion in the clinical practice and market. Herein, a binary chromatographic fingerprinting analysis method is developed via hydrophilic interaction chromatography and reversed-phase liquid chromatography to obtain more chemical information. Following that, an ultra-performance liquid chromatography with a triple quadrupole mass spectrometry method is furnished to simultaneously detect 17 ingredients of Angelicae Sinensis Radix gathered from six geographic zones in China. Eventually, the principal component analysis is successfully carried out to classify and differentiate the Angelicae Sinensis Radix from different origins, meanwhile the quantitative volcano plots was used to observe the changes of ingredient trends vividly. Accordingly, the proposed binary chromatography and triple quadrupole tandem mass spectrometry coupled with multivariate statistical analysis can be utilized as a facile and reliable method for origin tracing and quality control of Angelicae Sinensis Radix.
当归是一种闻名世界的中草药，原产于中国。由于许多环境和地理因素的影响，不同产地的当归在成分含量上可能存在差异，这给临床实践和市场带来了混乱。在此，我们开发了一种通过亲水作用色谱和反相液相色谱获得更多化学信息的二元色谱指纹分析方法。然后，我们提供了一种超高效液相色谱-三重四极杆质谱联用方法，同时检测来自中国六个地理区域的 17 种当归成分。最终，成功地进行了主成分分析，对不同产地的当归进行分类和区分，同时使用定量火山图生动地观察成分趋势的变化。因此，所提出的二元色谱和三重四极杆串联质谱结合多变量统计分析可以作为一种简单可靠的方法，用于当归的产地追溯和质量控制。
