Beijing Institute of Radiation Medicine, Beijing, China; China Pharmaceutical Culture Collection, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences, Institute of Lifeomics, Beijing, China.
Biomed Pharmacother. 2021 Jan;133:110954. doi: 10.1016/j.biopha.2020.110954. Epub 2020 Nov 29.
Anemarrhena asphodeloides is an herb widely used to treat symptoms associated with diabetes in traditional Chinese medicine. However, its key components and metabolites have low bioavailability and poor host absorption. To clarify the anti-diabetic mechanism of A. asphodeloides extract (AAE), we examined the anti-diabetic effects of AAE in rats with diabetes induced by a high-fat diet and streptozotocin. Faeces levels of the main components and metabolites of AAE were significantly higher than levels in plasma, which indicated that gut microbiota might play important roles in its anti-diabetic effect. Microbiological studies showed that unabsorbed components increased the diversity of the gut microbiota, enriched potentially beneficial bacteria, and suppressed potentially harmful bacteria. In vitro studies showed that AAE promoted the proliferation of Blautia coccoides, a bacterium with positive implication for diabetes, in a dose-dependent manner. AAE also promoted pancreatic cell regeneration and restored the function of pancreatic islet cells via peroxiredoxin 4 overexpression. Overall, these results suggest that AAE alleviates diabetes via modulating gut microbiota and protein expression.
知母是一种广泛用于治疗中医糖尿病相关症状的草药。然而，其主要成分和代谢物的生物利用度低，宿主吸收不良。为了阐明知母提取物（AAE）的抗糖尿病机制，我们研究了 AAE 对高脂肪饮食和链脲佐菌素诱导的糖尿病大鼠的抗糖尿病作用。AAE 的主要成分和代谢物在粪便中的水平明显高于血浆中的水平，这表明肠道微生物群可能在其抗糖尿病作用中发挥重要作用。微生物学研究表明，未被吸收的成分增加了肠道微生物群的多样性，富集了潜在有益的细菌，并抑制了潜在有害的细菌。体外研究表明，AAE 以剂量依赖的方式促进了糖尿病有益菌布劳特氏菌的增殖。AAE 还通过过氧化物酶 4 的过表达促进胰腺细胞的再生和胰岛细胞功能的恢复。总的来说，这些结果表明 AAE 通过调节肠道微生物群和蛋白质表达来缓解糖尿病。
