基于 MnO 纳米片调制的硫胺素发光测定丁酰胆碱酯酶活性。
Determination of butyrylcholinesterase activity based on thiamine luminescence modulated by MnO nanosheets.
机构信息
College of Chemistry, Jilin University, Changchun, 130012, PR China.
College of Electrical and Electronic Engineering, Changchun University of Technology, Changchun, 130012, PR China.
出版信息
Talanta. 2021 Mar 1;224:121831. doi: 10.1016/j.talanta.2020.121831. Epub 2020 Oct 31.
In this paper, a novel strategy for biosensing butyrylcholinesterase (BChE) activity is developed based on manganese dioxide (MnO) nanosheets to modulate the photoluminescence of thiamine (TH). The oxidase-like activity of MnO nanosheets enables them to catalyze the oxidation of non-fluorescent substrate TH to generate strong fluorescent thiochrome (TC). When the target BChE is introduced to form thiocholine in the presence of S-butyrylthiocholine iodide (BTCh), MnO nanosheets are reduced by thiocholine to Mn, resulting in the loss of their oxidase-like activity and the reduction of TC fluorescence. Based on this, a BChE activity fluorescence biosensor is constructed utilizing the luminescence behavior variation of TH and the oxidase-like activity of MnO nanosheets. The fluorescence biosensor shows a sensitive response to BChE, and the detection limit reaches 0.036 U L. In addition, the feasibility of the biosensor in real samples analysis is studied with satisfactory results.
本文提出了一种基于二氧化锰（MnO）纳米片来调节硫胺素（TH）光致发光的新型生物传感丁酰胆碱酯酶（BChE）活性的策略。MnO 纳米片的氧化酶样活性使其能够催化非荧光底物 TH 的氧化生成强荧光硫代色酮（TC）。当目标 BChE 存在于 S-丁酰硫代胆碱碘化物（BTCh）时，形成硫代胆碱，MnO 纳米片被硫代胆碱还原为 Mn，导致其氧化酶样活性丧失和 TC 荧光减少。基于此，利用 TH 的发光行为变化和 MnO 纳米片的氧化酶样活性构建了一种 BChE 活性荧光生物传感器。该荧光生物传感器对 BChE 具有灵敏的响应，检测限达到 0.036 U L。此外，还研究了该生物传感器在实际样品分析中的可行性，结果令人满意。
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