College of Chemistry, Jilin University, Changchun, 130012, PR China.
College of Electrical and Electronic Engineering, Changchun University of Technology, Changchun, 130012, PR China.
Talanta. 2021 Mar 1;224:121831. doi: 10.1016/j.talanta.2020.121831. Epub 2020 Oct 31.
In this paper, a novel strategy for biosensing butyrylcholinesterase (BChE) activity is developed based on manganese dioxide (MnO) nanosheets to modulate the photoluminescence of thiamine (TH). The oxidase-like activity of MnO nanosheets enables them to catalyze the oxidation of non-fluorescent substrate TH to generate strong fluorescent thiochrome (TC). When the target BChE is introduced to form thiocholine in the presence of S-butyrylthiocholine iodide (BTCh), MnO nanosheets are reduced by thiocholine to Mn, resulting in the loss of their oxidase-like activity and the reduction of TC fluorescence. Based on this, a BChE activity fluorescence biosensor is constructed utilizing the luminescence behavior variation of TH and the oxidase-like activity of MnO nanosheets. The fluorescence biosensor shows a sensitive response to BChE, and the detection limit reaches 0.036 U L. In addition, the feasibility of the biosensor in real samples analysis is studied with satisfactory results.
本文提出了一种基于二氧化锰（MnO）纳米片来调节硫胺素（TH）光致发光的新型生物传感丁酰胆碱酯酶（BChE）活性的策略。MnO 纳米片的氧化酶样活性使其能够催化非荧光底物 TH 的氧化生成强荧光硫代色酮（TC）。当目标 BChE 存在于 S-丁酰硫代胆碱碘化物（BTCh）时，形成硫代胆碱，MnO 纳米片被硫代胆碱还原为 Mn，导致其氧化酶样活性丧失和 TC 荧光减少。基于此，利用 TH 的发光行为变化和 MnO 纳米片的氧化酶样活性构建了一种 BChE 活性荧光生物传感器。该荧光生物传感器对 BChE 具有灵敏的响应，检测限达到 0.036 U L。此外，还研究了该生物传感器在实际样品分析中的可行性，结果令人满意。
