Tverdokhlebova Tamara S, Antipina Ludmila S, Kudryavtseva Valeriya L, Stankevich Ksenia S, Kolesnik Ilya M, Senokosova Evgenia A, Velikanova Elena A, Antonova Larisa V, Vasilchenko Dmitry V, Dambaev Georgiy T, Plotnikov Evgenii V, Bouznik Vyacheslav M, Bolbasov Evgeny N
Laboratory of Hybrid Plasma Systems, Tomsk Polytechnic University, Tomsk 634050, Russia.
Department of Hospital Surgery with the Course of Cardiovascular Surgery, Siberian State Medical University, Tomsk 634050, Russia.
Membranes (Basel). 2020 Dec 28;11(1):21. doi: 10.3390/membranes11010021.
Wound healing is a complex process and an ongoing challenge for modern medicine. Herein, we present the results of study of structure and properties of ferroelectric composite polymer membranes for wound healing. Membranes were fabricated by electrospinning from a solution of vinylidene fluoride/tetrafluoroethylene copolymer (VDF-TeFE) and polyvinylpyrrolidone (PVP) in dimethylformamide (DMF). The effects of the PVP content on the viscosity and conductivity of the spinning solution, DMF concentration, chemical composition, crystal structure, and conformation of VDF-TeFE macromolecules in the fabricated materials were studied. It was found that as PVP amount increased, the viscosity and conductivity of the spinning solutions decreased, resulting in thinner fibers. Using FTIR and XRD methods, it was shown that if the PVP content was lower than 50 wt %, the VDF-TeFE copolymer adopted a flat zigzag conformation (TTT conformation) and crystalline phases with ferroelectric properties were formed. Gas chromatography results indicated that an increase in the PVP concentration led to a higher residual amount of DMF in the material, causing cytotoxic effects on 3T3L1 fibroblasts. In vivo studies demonstrated that compared to classical gauze dressings impregnated with a solution of an antibacterial agent, ferroelectric composite membranes with 15 wt % PVP provided better conditions for the healing of purulent wounds.
伤口愈合是一个复杂的过程，也是现代医学面临的持续挑战。在此，我们展示了用于伤口愈合的铁电复合聚合物膜的结构和性能研究结果。通过静电纺丝法，从偏二氟乙烯/四氟乙烯共聚物（VDF-TeFE）和聚乙烯吡咯烷酮（PVP）在二甲基甲酰胺（DMF）中的溶液制备膜。研究了PVP含量对纺丝溶液的粘度和电导率、DMF浓度、制成材料中VDF-TeFE大分子的化学成分、晶体结构和构象的影响。发现随着PVP含量增加，纺丝溶液的粘度和电导率降低，导致纤维更细。使用傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）方法表明，如果PVP含量低于50 wt%，VDF-TeFE共聚物采用扁平锯齿构象（TTT构象）并形成具有铁电性质的晶相。气相色谱结果表明，PVP浓度增加导致材料中DMF残留量更高，对3T3L1成纤维细胞产生细胞毒性作用。体内研究表明，与浸渍有抗菌剂溶液的传统纱布敷料相比，含15 wt% PVP的铁电复合膜为脓性伤口愈合提供了更好的条件。
