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用妊娠血液中的抗体对胎盘抗原进行免疫荧光组织学定位(作者译)

[Immunofluorescent-histologic localization of a placenta-antigen with antibodies from pregnancy blood (author's transl)].

作者信息

Skrabei H, Meyer-Falk E, Mettler L

出版信息

Z Geburtshilfe Perinatol. 1977 Aug;181(4):264-8.

PMID:333799
Abstract

Placenta-specific antibodies were isolated by the incubation of washed placental tissue with pregancy serum and then desorption with citric acid pH3. The antigen responsible for the formation of the antibody could be localized via the immunofluorescence technique together with application of the placenta antibody in the syncytiotrophoblast layer. The antibodies are not complement-fixing and belong to the IgG class.

摘要

通过将洗涤后的胎盘组织与妊娠血清孵育,然后用pH3的柠檬酸解吸,分离出胎盘特异性抗体。通过免疫荧光技术以及在合体滋养层应用胎盘抗体,可以定位负责抗体形成的抗原。这些抗体不固定补体,属于IgG类。

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