Department of Medical Oncology, Ankara City Hospital, Sakarya, Turkey.
Department of Medical Oncology, Sakarya Education and Research Hospital, Sakarya, Turkey.
J Cancer Res Ther. 2020 Dec;16(Supplement):S128-S132. doi: 10.4103/jcrt.JCRT_891_18.
Helicobacter pylori(HP) is a significant causative agent of gastric cancer (GC). However, the underlying mechanisms involved in its pathogenesis and association with oncoproteins are unclear. The aim of the present study was to evaluate the relationship between HP infection and human epidermal growth factor receptor 2 (HER2) expression in GC patients.
Surgery (173) or endoscopic biopsy (35) specimen of 208 patients diagnosed with GC was evaluated for the presence of HER2 and HP. HER2 expression was assessed by fluorescence in situ hybridization (FISH) method, whereas HP status was evaluated histologically. Giemsa stain was used to identify HP status, in case HP could not be recognized in routine H and E-stained sections despite careful examination.
The median age was 63 years (27-91), and most patients were male (male/female: 149/59). Of all the 208 patients, HP was positive in 87 (41.8%) and negative in 121 (58.2%) patients. FISH positivity for HER2 was observed in 41 (19.7%) patients, whereas FISH negativity was observed in 167 (80.3%) patients. According to the Chi-square test, patient distribution was 21 in HER2-positive HP-negative group, 20 in HER2-positive HP-positive group, 100 in HER2-negative HP-negative group, and 67 in HER2-negative HP-positive group. No correlation was found between HP and HER2 status (P = 0.314). HP positivity had significant effect on median overall survival (27.4 vs. 12.9 months, P = 0.046).
Our results suggest that there is no relationship between HP infection and HER2 status in patients with GC.
幽门螺杆菌（HP）是胃癌（GC）的重要致病因子。然而，其发病机制及与癌蛋白的关系尚不清楚。本研究旨在评估 GC 患者中 HP 感染与人类表皮生长因子受体 2（HER2）表达之间的关系。
对 208 例经手术（173 例）或内镜活检（35 例）诊断为 GC 的患者的标本进行 HER2 和 HP 检测。HER2 表达采用荧光原位杂交（FISH）法检测，HP 状态采用组织学方法评估。如果在常规 H&E 染色切片中仔细检查仍无法识别 HP，则使用吉姆萨染色来识别 HP 状态。
中位年龄为 63 岁（27-91 岁），大多数患者为男性（男/女：149/59）。在所有 208 例患者中，87 例（41.8%）HP 阳性，121 例（58.2%）HP 阴性。41 例（19.7%）患者的 HER2 FISH 阳性，167 例（80.3%）患者的 HER2 FISH 阴性。卡方检验显示，HER2 阳性 HP 阴性组患者 21 例，HER2 阳性 HP 阳性组患者 20 例，HER2 阴性 HP 阴性组患者 100 例，HER2 阴性 HP 阳性组患者 67 例。HP 和 HER2 状态之间无相关性（P = 0.314）。HP 阳性对中位总生存期有显著影响（27.4 个月比 12.9 个月，P = 0.046）。
我们的研究结果表明，GC 患者中 HP 感染与 HER2 状态之间无相关性。
