Schoots A C, Gerlag P G, Mulder A W, Peeters J A, Cramers C A
Laboratory for Instrumental Analysis, Faculty of Chemical Engineering, Eindhoven University of Technology, The Netherlands.
Clin Chem. 1988 Jan;34(1):91-7.
Using "high performance" liquid chromatography, we studied non-protein-bound fractions and total concentrations of 18 solutes accumulating in sera from a group of 12 patients who were undergoing chronic ambulatory peritoneal dialysis (CAPD) and in predialysis sera from a group of 15 hemodialysis (HD) patients. We monitored longitudinal changes in solute concentrations for two patients with respect to change of therapy between HD and CAPD. The concentrations of pseudouridine (P less than 0.001), uric acid (P less than 0.001), and an unknown fluorescent solute, "UKF3" (P less than 0.01), differed in sera of HD and CAPD patients. When standardized with respect to serum creatinine concentrations, the concentration of the transfer-RNA catabolite, pseudouridine, was significantly (P less than 0.0001) higher in sera of CAPD patients than in HD patients, suggesting an increase in turnover of transfer RNA. In stepwise linear discriminant analysis, the combination of pseudouridine and the probably biochemically related fluorescent unknown, UKF3, contributed most to the differentiation between sera from CAPD and HD patients.
我们使用“高效”液相色谱法，研究了12例接受持续性非卧床腹膜透析（CAPD）患者血清中18种溶质的非蛋白结合部分和总浓度，以及15例血液透析（HD）患者透析前血清中这些溶质的情况。我们监测了2例患者在HD和CAPD治疗转换过程中溶质浓度的纵向变化。HD患者和CAPD患者血清中假尿苷（P<0.001）、尿酸（P<0.001）以及一种未知荧光溶质“UKF3”（P<0.01）的浓度存在差异。以血清肌酐浓度进行标准化后，CAPD患者血清中转录RNA分解代谢物假尿苷的浓度显著高于HD患者（P<0.0001），提示转录RNA周转率增加。在逐步线性判别分析中，假尿苷和可能与其生化相关的荧光未知物UKF3的组合对区分CAPD患者和HD患者的血清贡献最大。
