非特异性细胞毒性细胞在巴西日圆线虫感染中肠道上皮细胞损伤中的作用

Role of nonspecific cytotoxic cells in intestinal epithelial cell injury in Nippostrongylus brasiliensis infection.

作者信息

Hanglow A C, Perdue M H, Dyck N, Bienenstock J

机构信息

Intestinal Disease Research Unit, McMaster University, Hamilton, Ontario, Canada.

出版信息

Clin Immunol Immunopathol. 1988 Mar;46(3):406-11. doi: 10.1016/0090-1229(88)90059-1.

DOI:10.1016/0090-1229(88)90059-1

PMID:3338197

Abstract

We have employed a cultured crypt-like intestinal epithelial rat cell line (IEC-18) to assess whether killing by nonspecific cytotoxic cells contributes to epithelial cell destruction in Nippostrongylus brasiliensis (N.b.) infection. Spleen cells from N.b.-infected Sprague-Dawley rats, 11-13 days postinfection when villus atrophy and crypt hyperplasia were evident, killed IEC-18 in a 4-hr 51Cr-release assay. In contrast, intraepithelial leukocytes (IEL) from N.b.-infected rats showed decreased killing of IEC-18 compared to controls in a 16-hr assay. Spleen NK activity was increased in N.b.-infected rats whereas IEL NK activity was decreased. We conclude that direct cytotoxicity of crypt-like intestinal epithelial cells by nonspecific cytotoxic cells appears to play no significant role in intestinal injury in N.b. infection.

摘要

我们使用了一种培养的类隐窝肠上皮大鼠细胞系（IEC-18）来评估非特异性细胞毒性细胞的杀伤作用是否会导致巴西日圆线虫（N.b.）感染中上皮细胞的破坏。感染N.b.的Sprague-Dawley大鼠在感染后11 - 13天，此时绒毛萎缩和隐窝增生明显，其脾细胞在4小时的51Cr释放试验中杀死了IEC-18。相比之下，在16小时的试验中，感染N.b.大鼠的上皮内白细胞（IEL）对IEC-18的杀伤作用与对照组相比有所降低。感染N.b.的大鼠脾自然杀伤（NK）活性增加，而IEL的NK活性降低。我们得出结论，非特异性细胞毒性细胞对类隐窝肠上皮细胞的直接细胞毒性作用在N.b.感染所致的肠道损伤中似乎不起重要作用。