Department of Ophthalmology, University of Cologne, Cologne, Germany.
Department of Ophthalmology, HELIOS University Hospital Wuppertal, University Witten/Herdecke, Germany.
Histol Histopathol. 2020 Dec;35(12):1521-1531. doi: 10.14670/HH-18-300. Epub 2020 Dec 31.
To evaluate the presence and appearance of blood and lymphatic vessels in non-functioning bleb capsules of glaucoma drainage devices (GDD).
Non-functioning (n=14) GDD-bleb capsules of 12 patients were analyzed by immunohistochemistry for blood vessels (CD31, vascular endothelium), lymphatic vessels (lymphatic vessel endothelial hyaluronan receptor-1 [LYVE-1] and podoplanin) and macrophages (CD68).
CD31+++ blood vessels and CD68+ macrophages were detected in the outer layer of all specimens. LYVE-1 immunoreactivity was registered in single non-endothelial cells in 8 out of 14 (57%) bleb capsule specimens. Podoplanin-immunoreactivity was detected in all cases, located in cells and profiles of the collagen tissue network of the outer and/or the inner capsule layer. However, a colocalization of LYVE-1 and podoplanin as evidence for lymphatic vessels was not detected.
We demonstrate the presence of blood-vessels but absence of lymphatic vessels in non-functioning bleb capsules after GDD-implantation. While the absence of lymphatic vessels might indicate a possible reason for drainage device failure, this needs to be confirmed in upcoming studies, including animal experiments.
评估青光眼引流装置（GDD）无功能滤过泡囊中的血管和淋巴管的存在和形态。
对 12 名患者的 14 个无功能（n=14）GDD-滤过泡囊进行免疫组织化学分析，以检测血管（CD31、血管内皮细胞）、淋巴管（淋巴管内皮透明质酸受体-1[LYVE-1]和 podoplanin）和巨噬细胞（CD68）。
所有标本的外层均检测到 CD31+++血管和 CD68+巨噬细胞。在 14 个滤过泡囊标本中的 8 个（57%）中，LYVE-1 免疫反应性仅在单个非内皮细胞中被记录到。所有病例均检测到 podoplanin 免疫反应性，位于外层和/或内层囊层胶原组织网络中的细胞和轮廓中。然而，未检测到 LYVE-1 和 podoplanin 的共定位，这表明没有淋巴管的存在。
我们在 GDD 植入后无功能滤过泡囊中证实了血管的存在，但缺乏淋巴管。虽然淋巴管的缺失可能是引流装置失败的一个可能原因，但这需要在未来的研究中，包括动物实验来进一步证实。
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