State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha 410081, PR China.
Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Department of Biological and Environmental Engineering, Changsha 410022, PR China.
Comp Biochem Physiol C Toxicol Pharmacol. 2021 Mar;241:108966. doi: 10.1016/j.cbpc.2020.108966. Epub 2020 Dec 28.
Ferritin H can participate in the regulation of teleostean immunity. ORF sequences of RCC/WCC/WR-ferritin H were 609 bp, while WR-ferritin H gene possessed chimeric fragments or offspring-specific mutations. In order to elucidate regulation of immune-related signal transduction, three fibroblast-like cell lines derived from caudal fin of red crucian carp (RCC), white crucian carp (WCC) and their hybrid offspring (WR) were characterized and designated as RCCFCs, WCCFCs and WRFCs. A sharp increase of ferritin H mRNA was observed in RCCFCs, WCCFCs and WRFCs following lipopolysaccharide (LPS) challenge. Overexpression of RCC/WCC/WR-ferritin H can decrease MyD88-IRAK4 signal and antagonize NF-κB, TNFα promoter activity in RCCFCs, WCCFCs and WRFCs, respectively. These results indicated that ferritin H in hybrid offspring harbors highly-conserved domains with a close sequence similarity to those of its parents, playing a regulatory role in inflammatory signals.
铁蛋白 H 可参与调控硬骨鱼类的免疫。RCC/WCC/WR-铁蛋白 H 的 ORF 序列为 609bp，而 WR-铁蛋白 H 基因具有嵌合片段或后代特异性突变。为了阐明与免疫相关的信号转导的调控，我们从红鲫（RCC）、白鲫（WCC）及其杂交后代（WR）的尾鳍中分离出三种成纤维细胞系，并分别命名为 RCCFCs、WCCFCs 和 WRFCs。在 LPS 刺激后，RCCFCs、WCCFCs 和 WRFCs 中的铁蛋白 H mRNA 水平明显升高。过表达 RCC/WCC/WR-铁蛋白 H 可分别降低 RCCFCs、WCCFCs 和 WRFCs 中的 MyD88-IRAK4 信号和拮抗 NF-κB、TNFα 启动子活性。这些结果表明，杂交后代的铁蛋白 H 具有与其亲本高度保守的结构域，与亲本具有密切的序列相似性，在炎症信号中发挥调节作用。
