Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi-221005, India; Department of Zoology, School of Basic and Applied Sciences, Central University of Punjab, Bathinda-151001, India.
Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi-221005, India.
Ecotoxicol Environ Saf. 2021 Feb;209:111783. doi: 10.1016/j.ecoenv.2020.111783. Epub 2020 Dec 29.
Decabromodiphenyl ether (BDE-209) is widely used as a flame retardant in many products like electronic equipments, plastics, furniture and textiles. BDE-209, a thyroid hormones (THs)-disrupting chemical, affects male reproductive health through altered THs status in mouse model. The present study was designed in continuation to our earlier work to elucidate whether early life exposure to BDE-209 has a long term potential risk to male reproductive health. This study, therefore, aimed to evaluate the effect of maternal BDE-209 exposure during lactation and to elucidate possible mechanism(s) of its action on male reproduction in adult Parkes mice offspring. Lactating female Parkes mice were orally gavaged with 500, and 700 mg/kg body weight of BDE-209 in corn oil from postnatal day (PND) 1 to PND 28 along with 6-propyl-2-thiouracil (PTU)-treated positive controls and vehicle-treated controls. Male pups of lactating dams were euthanized at PND 75. Maternal BDE-209 exposure during lactation markedly affected histoarchitecture of testis and testosterone production with concomitant down-regulation in the expression of various steroidogenic markers in adult offspring. Maternal exposure to BDE-209 during lactation also interfered with germ cell dynamics and oxidative status in testes of adult mice offspring. A decreased expression of connexin 43 and androgen receptor was also evident in testes of these mice offspring; further, number, motility and viability of spermatozoa were also adversely affected in these mice. The results thus provide evidences that maternal exposure to BDE-209 during lactation causes reproductive toxicity in adult mice offspring.
十溴二苯醚（BDE-209）作为一种广泛应用于电子设备、塑料、家具和纺织品等多种产品的阻燃剂。BDE-209 是一种甲状腺激素（THs）干扰化学物质，通过改变小鼠模型中 THs 状态来影响男性生殖健康。本研究是在我们早期工作的基础上进行的，旨在阐明哺乳期暴露于 BDE-209 是否对男性生殖健康存在长期潜在风险。因此，本研究旨在评估哺乳期母体 BDE-209 暴露对雄性生殖的影响，并阐明其对成年 Parkes 小鼠后代生殖的作用机制。哺乳期的 Parkes 雌性小鼠从产后第 1 天（PND）到第 28 天，每天通过口服给予 500 和 700mg/kg 体重的 BDE-209 油溶液，同时给予丙基-2-硫代尿嘧啶（PTU）处理的阳性对照和载体处理的阴性对照。哺乳期母鼠的雄性幼仔在 PND 75 时处死。哺乳期母体 BDE-209 暴露明显影响睾丸的组织学结构和睾酮的产生，同时降低成年后代中各种类固醇生成标记物的表达。哺乳期母体暴露于 BDE-209 还干扰了成年小鼠后代睾丸中生殖细胞的动态平衡和氧化状态。这些小鼠后代睾丸中的连接蛋白 43 和雄激素受体的表达也明显减少；此外，这些小鼠的精子数量、活力和活力也受到不利影响。研究结果表明，哺乳期母体暴露于 BDE-209 会导致成年小鼠后代生殖毒性。
