Sorlozano-Puerto Antonio, Albertuz-Crespo Maria, Lopez-Machado Isaac, Gil-Martinez Lidia, Ariza-Romero Juan Jose, Maroto-Tello Alba, Baños-Arjona Alberto, Gutierrez-Fernandez Jose
Department of Microbiology, School of Medicine and PhD Program in Clinical Medicine and Public Health, University of Granada-ibs, Avda. de la Investigación, 11, 18016 Granada, Spain.
DMC Research Center, Camino de Jayena, 82, 18620 Alhendín, Spain.
Pharmaceuticals (Basel). 2020 Dec 29;14(1):21. doi: 10.3390/ph14010021.
Propyl-propane thiosulfinate (PTS) and propyl-propane thiosulfonate (PTSO) are two volatile compounds derived from with a widely documented antimicrobial activity. The aim of this study was to evaluate their anti-candidiasis activity and the ability of its gaseous phase to inhibit bacterial and yeast growth in vitro. The minimum inhibitory concentration of various antifungal products (including PTS and PTSO) was determined versus 203 clinical isolates of spp. through broth microdilution assay. Additionally, the antimicrobial activity through aerial diffusion of PTS and PTSO was evaluated over the growth of a collection of bacteria and yeasts cultivated in agar plates. All yeasts were susceptible to the antifungals tested, except and , that showed azole resistance. PTSO (MIC and MIC ranged from 4 to 16 mg/L and 8 to 32 mg/L, respectively) was significantly more active against yeasts than PTS (MIC and MIC ranged from 16 to 64 mg/L and 32 to 64 mg/L). Values were higher than those obtained for antifungal drugs. Gaseous phases of PTS and PTSO generated growth inhibition zones whose diameters were directly related to the substances concentration and inversely related to the microbial inoculum. The quantification of PTS and PTSO levels reached in the growth media through aerial diffusion displayed a concentration gradient from the central zone to the periphery. Only ATCC 27853 showed resistance, while yeasts ( ATCC 200955 and ATCC 6258) presented the higher susceptibility to both compounds. These results suggest that PTS and PTSO display antibacterial and anti-candidiasis activity in vitro through aerial diffusion, having potential use in human therapy.
丙基丙烷硫代亚磺酸盐（PTS）和丙基丙烷硫代磺酸盐（PTSO）是两种具有广泛抗菌活性记录的挥发性化合物。本研究的目的是评估它们的抗念珠菌病活性及其气相在体外抑制细菌和酵母生长的能力。通过肉汤微量稀释法测定了各种抗真菌产品（包括PTS和PTSO）对203株临床分离的 菌的最低抑菌浓度。此外，通过琼脂平板上培养的一系列细菌和酵母的生长来评估PTS和PTSO通过空气扩散的抗菌活性。除了显示对唑类耐药的 和 外，所有酵母对所测试的抗真菌剂均敏感。PTSO（MIC和MIC分别为4至16mg/L和8至32mg/L）对酵母的活性明显高于PTS（MIC和MIC分别为16至64mg/L和32至64mg/L）。这些值高于抗真菌药物的值。PTS和PTSO的气相产生了生长抑制区，其直径与物质浓度直接相关，与微生物接种量成反比。通过空气扩散在生长培养基中达到的PTS和PTSO水平的定量显示从中心区到周边的浓度梯度。只有ATCC 27853显示耐药，而酵母（ATCC 200955和ATCC 6258）对这两种化合物表现出更高的敏感性。这些结果表明，PTS和PTSO通过空气扩散在体外显示出抗菌和抗念珠菌病活性，在人类治疗中具有潜在用途。
