D'Amico Francesco, Casalino Gaia, Dinardo Francesca Rita, Schiavitto Michele, Camarda Antonio, Romito Diana, Bove Antonella, Circella Elena
Department of Veterinary Medicine, University of Bari "Aldo Moro", S. P. Casamassima km 3, 70010 Valenzano, BA, Italy.
Italian Rabbit Breeders Association-ANCI, Contrada Giancola snc, 71030 Volturara Appula, FG, Italy.
Vet Sci. 2023 Jun 23;10(7):411. doi: 10.3390/vetsci10070411.
Colibacillosis, caused by enteropathogenic (EPEC), is one of the most common diseases in rabbit farms, resulting in economic losses due to mortality and decrease in production. Until recently, antimicrobials were used to both treat and prevent disease on livestock farms, leading to the possible risk of antimicrobial resistance (AMR) and the selection of multidrug-resistant (MDR) bacteria. Therefore, interest in alternative control methods, such as the use of natural substances, has increased in the scientific community. The aim of this study was to evaluate the antimicrobial efficacy of Phyto-L (Pro Tech s.r.l.), a product containing organosulfur compounds (OSCs) such as propyl propane thiosulfonate (PTSO) from spp., against 108 strains of isolated from rabbits with colibacillosis from 19 farms. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Phyto-L were assessed. Bacterial suspensions with a charge of 10 CFU/mL, corresponding to those found in the rabbit gut under pathologic conditions, were tested with different concentrations from 20 to 0.15 μL/mL of Phyto-L. For each strain, the MIC and concentrations above the MIC were plated on Tryptic Soy agar (TSA) without Phyto-L to assess the MBCs. MIC and MBC values ranged from 1.25 to 5 μL/mL and 1.25 to 20 μL/mL, respectively, depending on the strain tested. The data showed an interesting antibacterial activity of Phyto-L against EPEC strains. Therefore, this product could be effective in preventing colibacillosis in field application, especially considering that 10-10 CFU/g of feces is the amount of usually found in the gut contents of rabbits under physiological condition.
由肠致病性大肠杆菌（EPEC）引起的大肠杆菌病是养兔场中最常见的疾病之一，会因死亡和产量下降造成经济损失。直到最近，抗菌药物仍被用于家畜养殖场的疾病治疗和预防，这导致了抗菌药物耐药性（AMR）以及多重耐药（MDR）细菌产生的潜在风险。因此，科学界对使用天然物质等替代控制方法的兴趣有所增加。本研究的目的是评估Phyto-L（Pro Tech s.r.l.公司生产）的抗菌效果，该产品含有有机硫化合物（OSC），如来自某菌种的丙基丙烷硫代磺酸盐（PTSO），用于对抗从19个养殖场患有大肠杆菌病的兔子中分离出的108株大肠杆菌。评估了Phyto-L的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。用不同浓度（20至0.15 μL/mL）的Phyto-L对电荷为10 CFU/mL的细菌悬液进行测试，该电荷对应于病理条件下兔肠道中发现的细菌悬液。对于每个菌株，将MIC及高于MIC的浓度接种在不含Phyto-L的胰蛋白胨大豆琼脂（TSA）上，以评估MBC。根据测试菌株的不同，MIC和MBC值分别在1.25至5 μL/mL和1.25至20 μL/mL之间。数据显示Phyto-L对EPEC菌株具有显著的抗菌活性。因此，该产品在实际应用中可能对预防大肠杆菌病有效，特别是考虑到生理条件下兔肠道内容物中通常发现的大肠杆菌数量为10⁸ - 10⁹ CFU/g粪便。
