Liou Jian-Chiun, Peng Chih-Wei, Basset Philippe, Chen Zhen-Xi
School of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan.
ESYCOM, Université Gustave Eiffel, CNRS, CNAM, ESIEE Paris, F-77454 Marne-la-Vallée, France.
Micromachines (Basel). 2020 Dec 29;12(1):25. doi: 10.3390/mi12010025.
The system designed in this study involves a three-dimensional (3D) microelectronic mechanical system chip structure using DNA printing technology. We employed diverse diameters and cavity thickness for the heater. DNA beads were placed in this rapid array, and the spray flow rate was assessed. Because DNA cannot be obtained easily, rapidly deploying DNA while estimating the total amount of DNA being sprayed is imperative. DNA printings were collected in a multiplexer driver microelectronic mechanical system head, and microflow estimation was conducted. Flow-3D was used to simulate the internal flow field and flow distribution of the 3D spray room. The simulation was used to calculate the time and pressure required to generate heat bubbles as well as the corresponding mean outlet speed of the fluid. The "outlet speed status" function in Flow-3D was used as a power source for simulating the ejection of fluid by the chip nozzle. The actual chip generation process was measured, and the starting voltage curve was analyzed. Finally, experiments on flow rate were conducted, and the results were discussed. The density of the injection nozzle was 50, the size of the heater was 105 μm × 105 μm, and the size of the injection nozzle hole was 80 μm. The maximum flow rate was limited to approximately 3.5 cc. The maximum flow rate per minute required a power between 3.5 W and 4.5 W. The number of injection nozzles was multiplied by 100. On chips with enlarged injection nozzle density, experiments were conducted under a fixed driving voltage of 25 V. The flow curve obtained from various pulse widths and operating frequencies was observed. The operating frequency was 2 KHz, and the pulse width was 4 μs. At a pulse width of 5 μs and within the power range of 4.3-5.7 W, the monomer was injected at a flow rate of 5.5 cc/min. The results of this study may be applied to estimate the flow rate and the total amount of the ejection liquid of a DNA liquid.
本研究设计的系统涉及一种采用DNA打印技术的三维（3D）微电子机械系统芯片结构。我们为加热器采用了不同的直径和腔体厚度。将DNA珠子放置在这个快速阵列中，并评估喷雾流速。由于DNA不易获取，在估计所喷射DNA总量的同时快速部署DNA至关重要。DNA打印件收集在一个多路复用器驱动微电子机械系统喷头中，并进行微流估计。使用Flow-3D模拟3D喷雾室的内部流场和流动分布。该模拟用于计算产生热气泡所需的时间和压力以及相应的流体平均出口速度。Flow-3D中的“出口速度状态”功能用作模拟芯片喷嘴喷射流体的动力源。测量了实际芯片生成过程，并分析了起始电压曲线。最后，进行了流速实验并讨论了结果。喷嘴密度为50，加热器尺寸为105μm×105μm，喷嘴孔尺寸为80μm。最大流速限制在约3.5cc。每分钟最大流速需要3.5W至4.5W之间的功率。喷嘴数量乘以100。在具有增大喷嘴密度的芯片上，在25V的固定驱动电压下进行实验。观察从各种脉冲宽度和工作频率获得的流动曲线。工作频率为2KHz，脉冲宽度为4μs。在脉冲宽度为5μs且功率范围为4.3 - 5.7W时，单体以5.5cc/min的流速注入。本研究结果可用于估计DNA液体的流速和喷射液体的总量。
