Sullivan M J, Conolly R B
Kresge Hearing Research Institute, Ann Arbor, Michigan.
Environ Res. 1988 Feb;45(1):64-70. doi: 10.1016/s0013-9351(88)80008-2.
The purpose of this investigation was to compare blood toluene levels in Sprague-Dawley rats after oral and inhalation administration. Groups of 30 rats were dosed by gavage with 86.7, 217, 433, or 867 mg toluene/kg body wt or exposed for up to 6 hr, 5 rats per exposure, to an atmosphere of either 200 or 1000 ppm toluene. Blood was sampled by cardiac puncture from 5 rats in each of the six dose groups at 0.5, 1.0, 2.0, 4.0, 6.0, and 24.0 hr after gavage dosing or the beginning of the inhalation exposure. Blood toluene levels were analyzed. A four-parameter model was fitted to the blood toluene levels of the orally dosed rats. The area under the curve generated by this model, representing total blood toluene concentration over 6 hr, was calculated and compared to the area under the blood toluene curve for the 6-hr inhalation exposure. Integrated areas from the two routes of exposure were used for direct comparison of oral and inhalation exposures. The data demonstrate that gavage dosing can be used to approximate inhalation exposure to toluene.
本研究的目的是比较经口给药和吸入给药后Sprague-Dawley大鼠的血液甲苯水平。将30只大鼠分为一组，通过灌胃给予86.7、217、433或867 mg甲苯/千克体重，或暴露于200或1000 ppm甲苯的环境中长达6小时，每次暴露5只大鼠。在灌胃给药或吸入暴露开始后的0.5、1.0、2.0、4.0、6.0和24.0小时，从六个剂量组中的每组5只大鼠通过心脏穿刺采集血液。分析血液甲苯水平。对经口给药大鼠的血液甲苯水平拟合四参数模型。计算该模型生成的曲线下面积，代表6小时内的总血液甲苯浓度，并与6小时吸入暴露的血液甲苯曲线下面积进行比较。来自两种暴露途径的积分面积用于直接比较经口暴露和吸入暴露。数据表明，灌胃给药可用于近似甲苯的吸入暴露。
