Gügler K, Funk C, Brodelius P
Institute of Biotechnology, ETH-Hönggerberg, Zürich, Switzerland.
Eur J Biochem. 1988 Jan 4;170(3):661-6. doi: 10.1111/j.1432-1033.1988.tb13748.x.
Tyrosine decarboxylase (EC 4.1.1.25) was induced in suspension cultures of Thalictrum rugosum by treatment with a yeast glucan elicitor. Maximum induction was observed at a carbohydrate concentration of 0.4 mg/g fresh weight of cells and maximum enzyme activity was reached 20 h after addition of elicitor. The enzyme was inducible in late exponential and early stationary growth phases. A good correlation between induced tyrosine decarboxylase activity and berberine biosynthesis has been established. It is suggested that tyrosine decarboxylase may be a key enzyme between primary and secondary metabolisms in the biosynthesis of norlaudanosoline-derived alkaloids.
用酵母葡聚糖激发子处理粗果唐松草悬浮培养物后，可诱导酪氨酸脱羧酶（EC 4.1.1.25）的产生。在碳水化合物浓度为0.4 mg/g细胞鲜重时观察到最大诱导效果，添加激发子后20小时达到最大酶活性。该酶在指数生长后期和稳定期早期可诱导产生。已确定诱导的酪氨酸脱羧酶活性与小檗碱生物合成之间具有良好的相关性。有人认为，酪氨酸脱羧酶可能是去甲劳丹碱衍生生物碱生物合成中初级代谢和次级代谢之间的关键酶。
