Brooks R F, Riddle P N
Imperial Cancer Research Fund Laboratories, Lincoln's Inn Fields, London, United Kingdom.
Exp Cell Res. 1988 Feb;174(2):378-87. doi: 10.1016/0014-4827(88)90308-4.
At low serum concentrations (3% or less), individual Swiss 3T3 cells display marked heterogeneity in proliferative capacity. Here we show that this heterogeneity arises at extremely high frequency within a clone, often with sister cells showing considerable differences in capacity for further proliferation. The heterogeneity is unlikely to be due to genetic instability or mutation. Instead, it appears to reflect physiological differences between cells in their requirement for serum growth factors. It is suggested that these differences arise because cells are unable to sustain production, at low growth factor concentrations, of some rare component which is itself required for growth factor action. We believe that the generation of heterogeneity in 3T3 cells has much in common with the phenomenon of senescence in diploid cells.
在低血清浓度（3%或更低）下，单个瑞士3T3细胞在增殖能力上表现出明显的异质性。在此我们表明，这种异质性在一个克隆内以极高的频率出现，常常姐妹细胞在进一步增殖能力上表现出显著差异。这种异质性不太可能是由于基因不稳定或突变。相反，它似乎反映了细胞在对血清生长因子需求方面的生理差异。有人提出，这些差异的产生是因为细胞在低生长因子浓度下无法维持某种本身是生长因子作用所必需的稀有成分的产生。我们认为3T3细胞中异质性的产生与二倍体细胞衰老现象有许多共同之处。
