生物炭添加对食物垃圾厌氧消化中产甲烷微生物群落的影响：生物炭中矿物质的作用。
Effect of biochar addition on microbial community and methane production during anaerobic digestion of food wastes: The role of minerals in biochar.
机构信息
Centre for Energy (M473), The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia; Faculty of Agricultural Technology, Universitas Brawijaya, Jalan Veteran, Malang, East Java 65154, Indonesia.
Centre for Energy (M473), The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
出版信息
Bioresour Technol. 2021 Mar;323:124585. doi: 10.1016/j.biortech.2020.124585. Epub 2020 Dec 21.
The effect of biochar addition on the microbial community and methane (CH) production during anaerobic digestion was experimentally investigated, focusing on the role of minerals in biochar. The biochar was prepared from pine sawdust by pyrolysis at 650 °C and 900 °C, respectively, and a subsample was leached with citric acid. The cultures with the addition of biochar, leached biochar, Fe, and leached biochar combined with Fe, respectively, were placed in bench-scale bioreactors for anaerobic digestion. Daily biogas production was measured by volume displacement method and analysed for CH concentration, which allowed the cumulative CH yield (Y) and daily CH production rate (R) to be determined. Culture samples were also taken daily for volatile fatty acids (VFAs) and microbial community analysis. Compared to the control without biochar addition, the addition of raw biochar significantly increased Y by 46.9% and R by 43.0%, while leached biochar only increased the Y by 33.2% and R by 18.2%, respectively. The Fe-containing minerals in biochar were found to enhance VFA degradation and increase population of Clostridia and Methanosaeta, improving the CH production.
研究了生物炭添加对厌氧消化过程中微生物群落和甲烷（CH）产生的影响，重点关注了生物炭中矿物质的作用。生物炭分别通过在 650°C 和 900°C 下热解松木锯末制备，并对亚样品用柠檬酸进行浸提。添加生物炭、浸提生物炭、Fe 和浸提生物炭与 Fe 结合的培养物分别置于台式生物反应器中进行厌氧消化。通过体积置换法测量每日沼气产量，并分析 CH 浓度，从而确定累积 CH 产率（Y）和每日 CH 产生率（R）。还每天采集培养物样品进行挥发性脂肪酸（VFAs）和微生物群落分析。与未添加生物炭的对照相比，添加原生物炭分别显著提高了 Y 值 46.9%和 R 值 43.0%，而浸提生物炭仅分别提高了 Y 值 33.2%和 R 值 18.2%。发现生物炭中的含铁矿物质可增强 VFA 降解，并增加产甲烷菌和产甲烷菌的数量，从而提高 CH 产量。
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