完成小鼠基底膜（IV型）胶原α1链核苷酸及推导氨基酸序列的cDNA克隆。

cDNA clones completing the nucleotide and derived amino acid sequence of the alpha 1 chain of basement membrane (type IV) collagen from mouse.

作者信息

Wood L, Theriault N, Vogeli G

机构信息

Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49001.

出版信息

FEBS Lett. 1988 Jan 18;227(1):5-8. doi: 10.1016/0014-5793(88)81402-9.

DOI:10.1016/0014-5793(88)81402-9

PMID:3338568

Abstract

Six cDNA clones add 3549 nucleotides to the DNA sequence of the alpha 1 chain of basement membrane (type IV) collagen. Thus the complete nucleotide and derived amino acid sequence of the alpha 1 type IV collagen with 5007 nucleotides coding for 1669 amino acids with a calculated Mr of 160,827 is known. The six cDNA clones cover the putative N-terminal signal peptide, the 7 S region and two thirds of the helical region extending into the previously published murine nucleotide sequence [(1986) Gene 43, 301]. The protein sequence for 289 amino acids of the helical region adjacent to the 7 S region has not previously been published for any species.

摘要

六个互补DNA（cDNA）克隆为基底膜（IV型）胶原蛋白α1链的DNA序列增加了3549个核苷酸。因此，编码1669个氨基酸、计算分子量为160827的α1（IV型）胶原蛋白的完整核苷酸序列和推导氨基酸序列是已知的。这六个cDNA克隆涵盖了假定的N端信号肽、7S区域以及延伸至先前发表的小鼠核苷酸序列[(1986) Gene 43, 301]中的三分之二螺旋区域。此前尚未有任何物种发表过与7S区域相邻的螺旋区域289个氨基酸的蛋白质序列。