肠道氨肽酶P的酶学性质：一种新的连续检测法
Enzymic properties of intestinal aminopeptidase P: a new continuous assay.
作者信息
Lasch J, Koelsch R, Steinmetzer T, Neumann U, Demuth H U
机构信息
Institute of Biochemistry, Medical Faculty, Martin-Luther-University Halle, GDR.
出版信息
FEBS Lett. 1988 Jan 25;227(2):171-4. doi: 10.1016/0014-5793(88)80891-3.
PMID:3338571
Abstract
A continuous photometric assay of aminopeptidase P activity was developed which is based on a coupled enzymic assay with the substrate Gly-Pro-Pro-pNA and DPP IV as auxiliary enzyme. This assay was used to evaluate the kinetic parameters and inhibitory profile of intestinal brush border aminopeptidase P.
摘要
基于以甘氨酰-脯氨酰-脯氨酸-对硝基苯胺为底物、二肽基肽酶IV为辅助酶的偶联酶法，开发了一种连续光度法来测定氨肽酶P的活性。该方法用于评估肠刷状缘氨肽酶P的动力学参数和抑制特性。
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