Jiang Li-Li, Liu Feng-Yi, Yang Wei, Li Chang-Li, Zhu Bao-Wei, Zhu Xiu-Hui
School of Chemical and Environmental Engineering, Yingkou Institute of Technology, Yingkou, 115014, Liaoning Province, China.
Jiangsu Key Laboratory for Biomass Energy and Material, Nanjing, 210042, Jiangsu Province, China.
Biotechnol Lett. 2021 Mar;43(3):711-717. doi: 10.1007/s10529-020-03063-0. Epub 2021 Jan 1.
To select a microbial consortium from intertidal sludge and evaluate its ability to convert crude glycerol from biodisel to high value-added products such as 1,3-propanediol (1,3-PDO) and lactic acid (LA).
A microbial consortium named CJD-S was selected from intertidal sludge and exhibited excellent performance for the conversion of crude glycerol to 1,3-PDO and LA. The composition of CJD-S was determined to be 85.99% Enterobacteriaceae and 13.75% Enterococcaceae by 16S rRNA gene amplicon high-throughput sequencing. In fed-batch fermentation with crude glycerol under nonsterile conditions, the highest concentrations of 1,3-PDO and LA were 41.47 g/L and 45.86 g/L, respectively.
The selected microbial consortium, CJD-S, effectively converted crude glycerol to 1,3-PDO and LA under nonsterile conditions and can contribute to the sustainable development of the biodiesel industry.
从潮间带污泥中筛选微生物群落，并评估其将生物柴油中的粗甘油转化为高附加值产品（如1,3 - 丙二醇（1,3 - PDO）和乳酸（LA））的能力。
从潮间带污泥中筛选出一个名为CJD - S的微生物群落，其在将粗甘油转化为1,3 - PDO和LA方面表现出优异性能。通过16S rRNA基因扩增子高通量测序确定CJD - S的组成，肠杆菌科占85.99%，肠球菌科占13.75%。在非无菌条件下以粗甘油进行分批补料发酵时，1,3 - PDO和LA的最高浓度分别为41.47 g/L和45.86 g/L。
所筛选的微生物群落CJD - S在非无菌条件下能有效地将粗甘油转化为1,3 - PDO和LA，有助于生物柴油行业的可持续发展。
