Dobiáš Radim, Havlíček Vladimír
Laboratory of Clinical Mycology, Institute of Health based in Ostrava, Department of Bacteriology and Mycology, Czech Republic, e-mail:
Klin Mikrobiol Infekc Lek. 2020 Jun;26(2):69-75.
Chromoblastomycosis and phaeohyphomycosis are less common fungal infections caused by dark-pigmented fungi. Virulence factors play an important role in the pathogenesis of these diseases. One of these factors, muriform cells, are the most important element for differential diagnosis of chromoblastomycosis and phaeohyphomycosis using clinical samples and various staining techniques. Accurate identification of pathogens causing chromoblastomycosis and phaeohyphomycosis is very important for correct and early antifungal therapy. Therefore, species identification of the etiological agent should be confirmed by sequencing of DNA from the culture. Early diagnosis may be crucial, especially in case of invasive forms of these infections. The diagnosis may be guided by some immunohistochemistry methods and DNA detection using polymerase chain reaction directly from clinical samples seems to be useful for identification of pathogens causing these severe and life-threatening infections.
着色芽生菌病和暗色丝孢霉病是由深色真菌引起的较罕见的真菌感染。毒力因子在这些疾病的发病机制中起重要作用。其中一个因子，砖隔状细胞，是使用临床样本和各种染色技术鉴别诊断着色芽生菌病和暗色丝孢霉病的最重要元素。准确鉴定引起着色芽生菌病和暗色丝孢霉病的病原体对于正确和早期的抗真菌治疗非常重要。因此，应通过对培养物中的DNA进行测序来确认病原体的种属鉴定。早期诊断可能至关重要，尤其是在这些感染的侵袭性形式中。诊断可由一些免疫组织化学方法指导，直接从临床样本中使用聚合酶链反应进行DNA检测似乎有助于鉴定引起这些严重且危及生命的感染的病原体。
