Shandong Provincial Key Laboratory of Animal Resistance Biology, Collaborative Innovation Center of Cell Biology in Universities of Shandong, College of Life Sciences, Institute of Biomedical Sciences, Shandong Normal University, Jinan, China.
J Clin Lab Anal. 2021 Mar;35(3):e23693. doi: 10.1002/jcla.23693. Epub 2021 Jan 2.
Diffuse large B-cell lymphoma (DLBCL) is the most common category of non-Hodgkin lymphoma (NHL). However, the underlying molecular mechanism of DLBCL remains unclear.
Real-time PCR and Western blot analysis were performed to assess the expression of ubiquitin-specific peptidase 21 (USP21) or enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2). CCK8 assay and cell death staining were carried out to examine the role of USP21 in cell proliferation and cell death, respectively.
We found that the deubiquitinase USP21 was highly expressed in the DLBCL lymphoid tissue. The expression of USP21 promoted DLBCL cell proliferation, while it had no obvious effect on cell death. In addition, we found that USP21 regulated cell proliferation via cysteine 221, the catalytic site of USP21. Furthermore, we identified that USP21 could stabilize EZH2, a protein required for germinal center formation and lymphoma formation.
The deubiquitinase USP21 promotes cell proliferation by maintaining the EZH2 protein level in DLBCL.
弥漫性大 B 细胞淋巴瘤（DLBCL）是非霍奇金淋巴瘤（NHL）中最常见的类型。然而，DLBCL 的潜在分子机制尚不清楚。
采用实时 PCR 和 Western blot 分析评估泛素特异性肽酶 21（USP21）或增强子的 zeste 2 多梳抑制复合物 2 亚基（EZH2）的表达。CCK8 检测和细胞死亡染色分别用于检测 USP21 在细胞增殖和细胞死亡中的作用。
我们发现去泛素酶 USP21 在 DLBCL 淋巴组织中高度表达。USP21 的表达促进了 DLBCL 细胞的增殖，而对细胞死亡没有明显影响。此外，我们发现 USP21 通过半胱氨酸 221（USP21 的催化位点）调节细胞增殖。此外，我们确定 USP21 可以稳定 EZH2，EZH2 是生发中心形成和淋巴瘤形成所必需的蛋白质。
去泛素酶 USP21 通过维持 DLBCL 中的 EZH2 蛋白水平促进细胞增殖。
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