Takabatake Reona, Onishi Mari, Mano Junichi, Kishine Masahiro, Soga Keisuke, Nakamura Kosuke, Kondo Kazunari, Kitta Kazumi
Food Research Institute, NARO.
FASMAC Co., Ltd.
Shokuhin Eiseigaku Zasshi. 2020;61(6):235-238. doi: 10.3358/shokueishi.61.235.
To quantify the amount of authorized GM maize or soybean, conversion factor (Cf) values are required for converting the copy number ratio of GM sequence to an endogenous sequence into weight-based GMO amounts. Cf values are available for the several latest real-time PCR instruments such as QuantStudio5, QuantStudio12K Flex, LightCycler 96, and LightCycler 480 for GM soybeans but not for GM maize. For the quantification of GM maize, we experimentally determined the Cf values targeting Cauliflower mosaic virus 35S promoter (P35S), GA21 construct specific, MIR604 event specific and MIR162 event specific sequences using the four real-time PCR instruments.
为了量化授权转基因玉米或大豆的数量，需要转换因子（Cf）值，以便将转基因序列与内源序列的拷贝数比转换为基于重量的转基因生物量。对于几种最新的实时荧光定量PCR仪器，如QuantStudio5、QuantStudio12K Flex、LightCycler 96和LightCycler 480，已有转基因大豆的Cf值，但转基因玉米没有。为了对转基因玉米进行定量，我们使用这四种实时荧光定量PCR仪器，通过实验确定了针对花椰菜花叶病毒35S启动子（P35S）、GA21构建体特异性、MIR604事件特异性和MIR162事件特异性序列的Cf值。
