作为SOX30的一个靶基因，对小鼠的雄性生育能力而言是可有可无的。
, a target gene of SOX30, is dispensable for male fertility in mice.
作者信息
Wang Fengsong, Kong Shuai, Hu Xuechun, Li Xin, Xu Bo, Yue Qiuling, Fu Kaiqiang, Ye Lan, Bai Shun
机构信息
School of Life Science, Anhui Medical University, Hefei, China.
Department of Urology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
出版信息
PeerJ. 2020 Dec 21;8:e10582. doi: 10.7717/peerj.10582. eCollection 2020.
BACKGROUND
The DNAJ family of molecular chaperones maintains protein homeostasis in mitotic and postmeiotic cells, especially germ cells. Recently, we found that the transcription factor SOX30 initiates transcription of during late meiosis and spermiogenesis in mouse testes.
METHODS
We used the CRISPR/Cas9 system to generate mutant mice and analyze the phenotype of the mutants.
RESULTS
Although is an evolutionarily conserved gene, it is not essential for spermatogenesis and male fertility. We provide this phenotypic information, which could prevent duplicative work by other groups.
背景
分子伴侣DNAJ家族在有丝分裂和减数分裂后细胞，尤其是生殖细胞中维持蛋白质稳态。最近，我们发现转录因子SOX30在小鼠睾丸减数分裂后期和精子发生过程中启动转录。
方法
我们使用CRISPR/Cas9系统生成突变小鼠并分析突变体的表型。
结果
尽管是一个进化上保守的基因，但它对精子发生和雄性生育力并非必需。我们提供了这一表型信息，这可以避免其他研究小组的重复工作。
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