通过 RNA-seq 技术对 17 种组织的小鼠转录组进行全面的转录组体图谱绘制。
A Comprehensive Mouse Transcriptomic BodyMap across 17 Tissues by RNA-seq.
机构信息
Center for Pharmacogenomics, School of Pharmacy, and State Key Laboratory of Genetic Engineering, School of Life Sciences and Shanghai Cancer Hospital/Cancer Institute, Fudan University, Shanghai, 200438, China.
Collaborative Innovation Center for Genetics and Development, Fudan University, Shanghai, 200438, China.
出版信息
Sci Rep. 2017 Jun 23;7(1):4200. doi: 10.1038/s41598-017-04520-z.
The mouse has been widely used as a model organism for studying human diseases and for evaluating drug safety and efficacy. Many diseases and drug effects exhibit tissue specificity that may be reflected by tissue-specific gene-expression profiles. Here we construct a comprehensive mouse transcriptomic BodyMap across 17 tissues of six-weeks old C57BL/6JJcl mice using RNA-seq. We find different expression patterns between protein-coding and non-coding genes. Liver expressed the least complex transcriptomes, that is, the smallest number of genes detected in liver across all 17 tissues, whereas testis and ovary harbor more complex transcriptomes than other tissues. We report a comprehensive list of tissue-specific genes across 17 tissues, along with a list of 4,781 housekeeping genes in mouse. In addition, we propose a list of 27 consistently and highly expressed genes that can be used as reference controls in expression-profiling analysis. Our study provides a unique resource of mouse gene-expression profiles, which is helpful for further biomedical research.
小鼠被广泛用作研究人类疾病、评估药物安全性和疗效的模式生物。许多疾病和药物作用表现出组织特异性，这种特异性可能反映在组织特异性基因表达谱中。在这里，我们使用 RNA-seq 构建了一个全面的 6 周龄 C57BL/6JJcl 小鼠 17 种组织的综合转录体 BodyMap。我们发现编码蛋白基因和非编码基因之间存在不同的表达模式。肝脏表达的转录组最简单，即肝脏在所有 17 种组织中检测到的基因数量最少，而睾丸和卵巢的转录组比其他组织更复杂。我们报告了一个全面的 17 种组织特异性基因列表，以及一个小鼠 4781 个管家基因列表。此外，我们提出了一个 27 个一致且高表达基因的列表，可作为表达谱分析中的参考对照基因使用。我们的研究提供了一个独特的小鼠基因表达谱资源，有助于进一步的生物医学研究。
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