Research Center for Coastal Environment Engineering Technology of Shandong Province, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong, China.
World J Microbiol Biotechnol. 2021 Jan 4;37(1):3. doi: 10.1007/s11274-020-02985-1.
Ampicillin sodium salt (AMP) is commonly and effectively used to prevent bacterial infection in algal culture, but the response of algal strains to AMP has not been investigated. In this study, Chlorella sorokiniana was selected to evaluate the influence of AMP on algae. AMP enhanced the contents of chlorophyll and two fatty acids, myristic acid (C22:1N9) and tetracosanoic acid (C6:0), but inhibited the growth, carotenoid production, and contents of 16 fatty acids in C. sorokiniana. A global transcriptome analysis from experimental data identified 3 825 upregulated and 1 432 downregulated differentially expressed genes (DEGs) in C. sorokiniana. The upregulated DEGs, such as hemB/alaD, mmaB/pduO, cox15/ctaA, fxN, cpoX/hemF, and earS/gltX, were enriched in the porphyrin and chlorophyll metabolism pathways, whereas the downregulated DEGs, including lcyB (crtL1), crtY (lcyE, crtL2), lut1 (CYP97C1), z-isO, crtZ and crtisO (crtH), were enriched in the carotenoid biosynthesis pathway, and the downregulated DEGs, abH, fadD, fabF, acsL, fabG, and accD were enriched in the fatty acid biosynthesis pathway. Thus, the use of AMP to obtain an axenic strain revealed that AMP might affect the regulatory dynamics and the results of the metabolic process in C. sorokiniana. The data obtained in the study provide foundational information for algal purification and aseptic processing.
氨苄青霉素钠盐（AMP）通常被有效地用于预防藻类培养中的细菌感染，但藻类菌株对 AMP 的反应尚未得到研究。在这项研究中，选择了小球藻来评估 AMP 对藻类的影响。AMP 提高了叶绿素和两种脂肪酸（肉豆蔻酸（C22：1N9）和二十四烷酸（C6：0））的含量，但抑制了小球藻的生长、类胡萝卜素的产生以及 16 种脂肪酸的含量。来自实验数据的全球转录组分析鉴定了小球藻中 3825 个上调和 1432 个下调的差异表达基因（DEGs）。上调的 DEGs，如 hemB/alaD、mmaB/pduO、cox15/ctaA、fxN、cpoX/hemF 和 earS/gltX，富集在卟啉和叶绿素代谢途径中，而下调的 DEGs，包括 lcyB（crtL1）、crtY（lcyE、crtL2）、lut1（CYP97C1）、z-isO、crtZ 和 crtisO（crtH），富集在类胡萝卜素生物合成途径中，而下调的 DEGs，abH、fadD、fabF、acsL、fabG 和 accD，富集在脂肪酸生物合成途径中。因此，使用 AMP 获得无菌株的结果表明，AMP 可能会影响小球藻的调控动态和代谢过程的结果。本研究获得的数据为藻类的纯化和无菌处理提供了基础信息。
