Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015, China.
Inflammation. 2021 Jun;44(3):1096-1107. doi: 10.1007/s10753-020-01405-8. Epub 2021 Jan 4.
To study the effects of betulin (BE) on myocardial ischemia-reperfusion (I/R) injury in rats, electrocardiogram (ECG) was detected by an electrocardiograph; myocardial infarction was evaluated by triphenyltetrazolium (TTC) staining, serum biochemical indicators myocardial enzymes creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), serum superoxide dismutase (SOD), glutathione (GSH), nitric oxide (NO), and malondialdehyde (MDA); and inflammatory cytokines were tested by using commercial kits. The expression of the Siti1/NLRP3/NF-κB signaling pathway was detected by western blotting and immunohistochemistry experiments. BE improved ECG; reduced myocardial infarction area; decreased CK, LDH, AST, MDA, NO, and inflammatory cytokines; and increased SOD and GSH in I/R rats. In addition, BE also increased Siti1 and decreased the NLRP3/NF-κB signaling pathway in I/R rats. This study shows that the protection of BE is associated with changes in the Siti1/NLRP3/NF-κB pathway.
为了研究白桦脂醇（BE）对大鼠心肌缺血再灌注（I/R）损伤的影响，采用心电图仪检测心电图；采用三苯基四氮唑（TTC）染色评估心肌梗死；通过商业试剂盒检测血清生化指标心肌酶肌酸激酶（CK）、乳酸脱氢酶（LDH）、天门冬氨酸氨基转移酶（AST）、血清超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、一氧化氮（NO）和丙二醛（MDA）；采用 Western blot 和免疫组化实验检测 Siti1/NLRP3/NF-κB 信号通路的表达。BE 改善了 ECG；减少了 I/R 大鼠的心肌梗死面积；降低了 CK、LDH、AST、MDA、NO 和炎性细胞因子；并增加了 I/R 大鼠的 SOD 和 GSH。此外，BE 还增加了 Siti1，降低了 I/R 大鼠的 NLRP3/NF-κB 信号通路。本研究表明，BE 的保护作用与 Siti1/NLRP3/NF-κB 通路的变化有关。
