Orlowski J, Bird C E, Clark A F
Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.
J Endocrinol. 1988 Jan;116(1):81-90. doi: 10.1677/joe.0.1160081.
Androgen metabolism and the regulation of rat ventral prostate cell proliferation and secretory function were examined during sexual maturation. Changes in acid phosphatase (AP) characteristics were measured as a marker of androgen-dependent prostatic secretory function. In immature (21-day-old) rats, total AP activity per cell was low (14.2 +/- 1.3 mol p-nitrophenol phosphate hydrolysed/h per mg DNA); it increased threefold as the weight, protein and DNA contents of the prostate increased to adult (65-day) levels. This corresponded with significant (P less than 0.001) increases in the staining intensities of three of the four bands of secretory AP on isoelectric focusing gels. The extent of inhibition of AP by tartrate decreased at the same time. Secretory AP is known to be relatively tartrate-resistant. The changes in AP activity occurred after prostatic 5 alpha-dihydrotestosterone (5 alpha-DHT) levels increased from 4.6 +/- 0.7 pmol/mg DNA (21 days) to reach a peak of 17.6 +/- 2.3 pmol/mg DNA at 58 days. Prostatic 5 alpha-DHT concentrations were always higher than testosterone levels. Prostatic 5 alpha-androstane-3 alpha,17 beta-diol (3 alpha-Adiol) levels were lower than 5 alpha-DHT levels except on day 58 when levels peaked dramatically at 26.2 +/- 5.5 pmol/mg DNA. Changes in prostatic 5 alpha-DHT and 3 alpha-Adiol levels corresponded with changes in 5 alpha-reductase and 3 alpha-hydroxysteroid oxidoreductase (3 alpha-HSOR) activities. The oxidative reaction of 3 alpha-HSOR was approximately fourfold higher than the reductive reaction, indicating a preference for the formation of 5 alpha-DHT. The plasma levels of testosterone, 5 alpha-DHT and 3 alpha-Adiol cannot account for their respective prostatic levels, indicating the importance of the steroid-metabolizing enzymes in regulating intracellular androgen levels. Changes in the AP characteristics could be correlated with the androgen status of the prostate.
在性成熟过程中，对雄激素代谢以及大鼠腹侧前列腺细胞增殖和分泌功能的调节进行了研究。测量酸性磷酸酶（AP）特性的变化，作为雄激素依赖性前列腺分泌功能的标志物。在未成熟（21日龄）大鼠中，每个细胞的总AP活性较低（每毫克DNA每小时水解14.2±1.3摩尔对硝基苯磷酸）；随着前列腺的重量、蛋白质和DNA含量增加到成年（65日龄）水平，其活性增加了三倍。这与等电聚焦凝胶上分泌性AP的四条带中的三条带的染色强度显著（P小于0.001）增加相对应。同时，酒石酸盐对AP的抑制程度降低。已知分泌性AP相对耐酒石酸盐。AP活性的变化发生在前列腺5α-双氢睾酮（5α-DHT）水平从4.6±0.7皮摩尔/毫克DNA（21天）增加到58天时达到峰值17.6±2.3皮摩尔/毫克DNA之后。前列腺5α-DHT浓度始终高于睾酮水平。前列腺5α-雄烷-3α,17β-二醇（3α-二醇）水平低于5α-DHT水平，除了在58天时，其水平急剧峰值达到26.2±5.5皮摩尔/毫克DNA。前列腺5α-DHT和3α-二醇水平的变化与5α-还原酶和3α-羟基类固醇氧化还原酶（3α-HSOR）活性的变化相对应。3α-HSOR的氧化反应比还原反应高约四倍，表明更倾向于形成5α-DHT。睾酮、5α-DHT和3α-二醇的血浆水平不能解释它们各自在前列腺中的水平，表明类固醇代谢酶在调节细胞内雄激素水平方面的重要性。AP特性的变化与前列腺的雄激素状态相关。
