Food Ingredients & Technology Institute, R&D Division, Morinaga Milk Industry Co., Ltd., 5-1-83 Higashihara, Zama, Kanagawa 252-8583, Japan.
R&D Management Department, Morinaga Milk Industry Co., Ltd., 5-1-83 Higashihara, Zama, Kanagawa 252-8583, Japan.
Benef Microbes. 2021 Feb 24;12(1):43-53. doi: 10.3920/BM2020.0100. Epub 2021 Jan 4.
The genus comprises various bacterial species, and the complement of species within the human intestinal tract differs from individual to individual. The balance of these bifidobacterial species remains poorly understood, although it is known that the abundance of bifidobacteria increases following the ingestion of prebiotics. We previously conducted a randomised, placebo-controlled, double-blind, crossover study of 2 g/day lactulose ingestion for 2 weeks in 60 Japanese women. To study the effect of lactulose ingestion on each bifidobacterial species, here, we measured the abundance of each of the principal bifidobacterial species. After lactulose ingestion, the log cell counts of the group (8.97±0.08 vs 9.39±0.08, =0.0019), group (9.45±0.10 vs 9.65±0.10, =0.0032) and group (9.01±0.07 vs 9.29±0.07, =0.0012) were significantly higher than in the placebo ingestion control group. However, the log cell counts were similar for (8.12±0.12 vs 8.33±0.12, =0.20), (9.08±0.12 vs 9.42±0.14, =0.095) and subspecies (8.65±0.53 vs 8.46±0.46, =0.77). Cluster analysis of the log cell count data at the bifidobacterial species level revealed three distinct clusters, but the combinations and ratios of the constituent bifidobacteria were not affected by lactulose ingestion. Furthermore, principal coordinate analysis of the intestinal microbiota in the lactulose and placebo ingestion groups using Illumina MiSeq showed no significant differences in the intestinal microbiota as a whole. These results suggest that 2 g/day lactulose ingestion for 2 weeks significantly increases the abundance of intestinal bifidobacteria, but does not affect the intestinal microbiota as a whole.
该属包含各种细菌物种，而人类肠道内的物种组成因个体而异。双歧杆菌物种的平衡仍知之甚少，尽管已知摄入益生元后双歧杆菌的丰度会增加。我们之前进行了一项随机、安慰剂对照、双盲、交叉研究，在 60 名日本女性中每天摄入 2 克乳果糖，持续 2 周。为了研究乳果糖摄入对每种双歧杆菌的影响，我们在这里测量了主要双歧杆菌的丰度。在乳果糖摄入后， 组（8.97±0.08 与 9.39±0.08，=0.0019）、 组（9.45±0.10 与 9.65±0.10，=0.0032）和 组（9.01±0.07 与 9.29±0.07，=0.0012）的对数细胞计数明显高于安慰剂摄入对照组。然而， （8.12±0.12 与 8.33±0.12，=0.20）、 （9.08±0.12 与 9.42±0.14，=0.095）和 亚种 （8.65±0.53 与 8.46±0.46，=0.77）的对数细胞计数相似。双歧杆菌物种水平的对数细胞计数数据的聚类分析显示了三个不同的簇，但乳果糖摄入并没有影响组成双歧杆菌的组合和比例。此外，使用 Illumina MiSeq 对乳果糖和安慰剂摄入组的肠道微生物群进行主坐标分析显示，整个肠道微生物群没有显著差异。这些结果表明，2 克/天乳果糖连续摄入 2 周可显著增加肠道双歧杆菌的丰度，但不会影响整个肠道微生物群。
