Lei Ting, Qian Hu, Lei Pengfei, Hu Yihe
Department of Orthopeadic Surgery, Xiangya Hospital Central South University, China.
Biomater Sci. 2021 Feb 21;9(4):1409-1420. doi: 10.1039/d0bm01555e. Epub 2021 Jan 4.
Tantalum (Ta) implants fabricated by current processing techniques inevitably contain more or less oxygen impurities due to the extremely high melting point and high affinity of oxygen for Ta. Therefore, in this study we investigated whether oxygen impurities cause any effects on the bioactivity of Ta. EDS analysis demonstrated the surface oxygen content difference among different fabricated Ta samples, and the surface water contact angle (WCA) of Ta with high oxygen content (HO-Ta) was significantly higher than that of Ta with medium (MO-Ta) and low (LO-Ta) oxygen content. The in vitro cellular experiments showed that MC3T3-E1 cells on Ta with lower oxygen content exhibited better adhesion, growth, morphological development and in vitro osteogenic ability. Similarly, the in vivo animal experiments indicated the better bone regeneration and ingrowth performances of Ta with lower oxygen content. In addition, the highest ROS production was detected in the HO-Ta group, while the lowest in the LO-Ta group. This study suggests that the oxygen content within Ta, which occurs unavoidably due to technical limitations, negatively affects the bioactivity of Ta in a dose-dependent manner, indicating the need to develop techniques to produce orthopedic all-Ta implants.
由于钽（Ta）的熔点极高且氧对Ta具有高亲和力，采用当前加工技术制造的钽植入物不可避免地或多或少含有氧杂质。因此，在本研究中，我们调查了氧杂质是否会对Ta的生物活性产生任何影响。能谱分析表明不同制造工艺的Ta样品表面氧含量存在差异，高氧含量Ta（HO-Ta）的表面水接触角（WCA）显著高于中等氧含量Ta（MO-Ta）和低氧含量Ta（LO-Ta）。体外细胞实验表明，氧含量较低的Ta上的MC3T3-E1细胞表现出更好的黏附、生长、形态发育和体外成骨能力。同样，体内动物实验表明氧含量较低的Ta具有更好的骨再生和向内生长性能。此外，HO-Ta组检测到的活性氧生成量最高，而LO-Ta组最低。本研究表明，由于技术限制不可避免地存在于Ta中的氧含量以剂量依赖方式对Ta的生物活性产生负面影响，这表明需要开发生产全钽骨科植入物的技术。
