Rutka J T, Hall J, Giblin J R, Dougherty D V, Edwards M S, Stern R, Rosenblum M L
Department of Neurological Surgery, School of Medicine, University of California, San Francisco.
J Neurosurg. 1988 Feb;68(2):251-8. doi: 10.3171/jns.1988.68.2.0251.
To determine how medulloblastoma cells might influence the proliferation and phenotype of normal stromal cells, normal human leptomeningeal cells were treated in culture with medulloblastoma-conditioned medium; their ability to incorporate tritiated thymidine and synthesize collagen was measured. The treated leptomeningeal cells had a significantly greater uptake of tritiated thymidine and grew faster than control leptomeningeal cells. Immunofluorescence studies demonstrated a greater intensity of staining for procollagen type III in the cell layer of the treated cultures than in control cultures; diethylaminoethyl (DEAE)-cellulose chromatography of the medium showed that the treated cells synthesized predominantly type III collagen, whereas control cells synthesized type I collagen. Analysis of the medulloblastoma-conditioned medium revealed that the soluble factor responsible for these effects in an acid- and heat-stable protein. The increased proliferation and altered collagen synthesis induced in leptomeningeal cell cultures by a soluble factor from a medulloblastoma are examples of how tumor and stromal elements interact, and may be related to the process of desmoplasia often observed in medulloblastomas in vivo.
为了确定髓母细胞瘤细胞如何影响正常基质细胞的增殖和表型，将正常人软脑膜细胞在培养中用髓母细胞瘤条件培养基处理；测量它们摄取氚标记胸腺嘧啶核苷和合成胶原蛋白的能力。处理后的软脑膜细胞对氚标记胸腺嘧啶核苷的摄取显著增加，并且比对照软脑膜细胞生长得更快。免疫荧光研究表明，处理后的培养物细胞层中III型前胶原的染色强度高于对照培养物；培养基的二乙氨基乙基（DEAE）-纤维素色谱显示，处理后的细胞主要合成III型胶原，而对照细胞合成I型胶原。对髓母细胞瘤条件培养基的分析表明，导致这些效应的可溶性因子是一种耐酸且耐热的蛋白质。髓母细胞瘤的可溶性因子在软脑膜细胞培养物中诱导的增殖增加和胶原蛋白合成改变，是肿瘤和基质成分相互作用的实例，并且可能与在体内髓母细胞瘤中经常观察到的促结缔组织增生过程有关。
