Gupta S K, Gilbert A B, Walker M A
AFRC Institute of Animal Physiology and Genetics Research, Roslin, Midlothian, U.K.
J Reprod Fertil. 1988 Jan;82(1):219-25. doi: 10.1530/jrf.0.0820219.
Semi-serial (1 in 20) sections of ovaries were studied and only two types of atresia were identified--non-bursting and bursting. Smaller, non-yolky follicles (less than 1 mm diameter) showed non-bursting atresia. Atresia in follicles greater than 1 mm diameter was invariably of the bursting type which involved the rupture of the follicular wall, and the extrusion of yolk and cellular debris through the rupture site into the stroma. However, this rupture site was small and consequently was not visible in every section but it could always be seen when the follicle was followed in semi-serial sections. The mitotic index of granulosa cells in bursting atretic follicles was much lower than that for normal follicles. The most common criteria for distinguishing non-bursting atretic follicles were the extremely shrunken, irregularly shaped oocytes and the separation of the granulosa from the theca. In bursting atretic follicles, reliable indications were the presence in the ooplasm of some cells or cellular debris, and disorganization of the yolk and granulosa tissue. The presence of pycnotic nuclei in the granulosa cells was not a consistent feature of all atretic follicles of the hen.
对卵巢的半连续切片（20张切片中的1张）进行了研究，仅识别出两种闭锁类型——非破裂型和破裂型。较小的、无卵黄的卵泡（直径小于1毫米）表现为非破裂型闭锁。直径大于1毫米的卵泡闭锁总是破裂型的，这涉及卵泡壁的破裂，以及卵黄和细胞碎片通过破裂部位挤入间质。然而，这个破裂部位很小，因此并非在每张切片中都可见，但在半连续切片中追踪卵泡时总能看到。破裂型闭锁卵泡中颗粒细胞的有丝分裂指数远低于正常卵泡。区分非破裂型闭锁卵泡最常见的标准是极度萎缩、形状不规则的卵母细胞以及颗粒细胞与卵泡膜的分离。在破裂型闭锁卵泡中，可靠的指征是卵质中存在一些细胞或细胞碎片，以及卵黄和颗粒组织紊乱。颗粒细胞中存在固缩核并非母鸡所有闭锁卵泡的一致特征。
