School of Life Science, Zhengzhou University, Zhengzhou, Henan Province, China; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan 455000, China.
School of Life Science, Zhengzhou University, Zhengzhou, Henan Province, China; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Ecotoxicol Environ Saf. 2021 Jan 15;208:111680. doi: 10.1016/j.ecoenv.2020.111680. Epub 2020 Nov 25.
The widespread commercialization of genetically modified (GM) cotton makes it important to assess the potential impact of this recombinant crop on non-target organisms. As important natural enemies of cotton field predators, green lacewing Chrysoperla sinica larvae are exposed to Bt insecticidal proteins expressed by GM cotton by feeding on herbivorous pests, and adults are directly exposed to Bt proteins by cotton pollen consumption. However, potential impacts of transgenic Bt cotton on C. sinica remain unclear. In this study, we evaluated the effects of two transgenic cotton varieties, CCRI41 and CCRI45, which express Cry1Ac (Bt toxin) and CpTI (Cowpea Trypsin Inhibitor), on C. sinica larvae and adults. After being fed with cotton aphids Aphis gossypii reared on transgenic cotton, the survival rate, developmental duration, pupation rate, and emergence rate of larvae were not adversely affected. After being fed two types of transgenic cotton pollen, the 7-day weight of adults and the preoviposition period and the cumulative oviposition of females were not significantly different from control specimen. Taken together, these results indicate that the potential risks of the two tested GM cotton varieties for the predator C. sinica are negligible. CAPSULE: Our study indicated that GM cotton varieties CCRI41 and CCRI45 have no adverse effects on insect predator C. sinica.
广泛商业化的转基因（GM）棉花使得评估这种重组作物对非目标生物的潜在影响变得重要。绿蝽 Chrysoperla sinica 幼虫是棉花田捕食性天敌的重要代表，它们通过取食植食性害虫而接触到由 GM 棉花表达的 Bt 杀虫蛋白，成虫则通过取食棉花花粉直接暴露于 Bt 蛋白。然而，转基因 Bt 棉花对 C. sinica 的潜在影响仍不清楚。在这项研究中，我们评估了表达 Cry1Ac（Bt 毒素）和 CpTI（豇豆胰蛋白酶抑制剂）的两种转基因棉花品种 CCRI41 和 CCRI45 对 C. sinica 幼虫和成虫的影响。在取食以转基因棉花为食的棉蚜 Aphis gossypii 后，幼虫的存活率、发育持续时间、化蛹率和羽化率不受不利影响。取食两种类型的转基因棉花花粉后，成虫的 7 天体重以及雌虫的预产卵期和累计产卵量与对照标本没有显著差异。综上所述，这些结果表明，两种测试的 GM 棉花品种对捕食性天敌 C. sinica 的潜在风险可以忽略不计。
