吉斯索生物碱，一种来自树皮的新生物碱。
Geissospermiculatine, a New Alkaloid from Bark.
机构信息
Department of Physical Chemistry, Faculty of Pharmacy with Laboratory Medicine Division, Medical University of Warsaw, 02-097 Warsaw, Poland.
Department of Physiology and Pharmacology, Karolinska Institutet, 17165 Stockholm, Sweden.
出版信息
Molecules. 2020 Dec 31;26(1):143. doi: 10.3390/molecules26010143.
A new alkaloid, geissospermiculatine was characterized in A. H. Gentry bark (Apocynaceae). Here, following a simplified isolation protocol, the structure of the alkaloid was elucidated through GC-MS, LC-MS/MS, 1D, and 2D NMR (COSY, ROESY, HSQC, HMBC, H-N HMBC). Cytotoxic properties were evaluated in vitro on malignant THP-1 cells, and the results demonstrated that the cytotoxicity of the alkaloid (30 μg/mL) was comparable with staurosporine (10 μM). Additionally, the toxicity was tested on zebrafish () embryos in vivo by monitoring their development (0-72 h); toxicity was not evident at 30 μg/mL.
从 A. H. 根皮（夹竹桃科）中分离得到一种新的生物碱，吉斯索斯佩米林碱。在此，通过简化的分离方案，利用 GC-MS、LC-MS/MS、1D 和 2D NMR（COSY、ROESY、HSQC、HMBC、H-N HMBC）阐明了该生物碱的结构。体外评估了其对恶性 THP-1 细胞的细胞毒性，结果表明该生物碱（30μg/mL）的细胞毒性与星孢菌素（10μM）相当。此外，还通过监测斑马鱼（Danio rerio）胚胎的发育（0-72 h），在体内评估了该生物碱的毒性；在 30μg/mL 时，未观察到毒性。
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