Genetics Area, Faculty of Marine and Environmental Sciences, INMAR, University of Cadiz, 11510 Cádiz, Spain.
Genes (Basel). 2020 Dec 31;12(1):49. doi: 10.3390/genes12010049.
aquaculture production has experienced a great increase in the last decade and, consequently, the genome knowledge of the species is gaining attention. In this sense, obtaining a high-density genome mapping of the species could offer clues to the aquaculture improvement in those aspects not resolved so far. In the present article, a review and new processed data have allowed to obtain a high-density BAC-based cytogenetic map of beside the analysis of the sequences of such BAC clones to achieve integrative data. A total of 93 BAC clones were used to localize the chromosome complement of the species and 588 genes were annotated, thus almost reaching the 2.5% of the genome sequences. As a result, important data about its genome organization and evolution were obtained, such as the lesser gene density of the large metacentric pair compared with the other metacentric chromosomes, which supports the theory of a sex proto-chromosome pair. In addition, chromosomes with a high number of linked genes that are conserved, even in distant species, were detected. This kind of result widens the knowledge of this species' chromosome dynamics and evolution.
在过去的十年中，水产养殖产量有了很大的增长，因此，物种的基因组知识受到了关注。从这个意义上说，获得该物种的高密度基因组图谱可能为目前尚未解决的水产养殖改良提供线索。在本文中，通过回顾和新处理的数据，获得了一个高密度基于 BAC 的细胞学图谱， 并对这些 BAC 克隆的序列进行了分析，以获得综合数据。总共使用了 93 个 BAC 克隆来定位该物种的染色体组，注释了 588 个基因，几乎达到了 基因组序列的 2.5%。结果得到了关于其基因组组织和进化的重要数据，例如，与其他着丝粒染色体相比，大着丝粒染色体对的基因密度较小，这支持了性原染色体对的理论。此外，还检测到了具有大量连锁基因的染色体，这些基因甚至在遥远的物种中也是保守的。这种结果拓宽了对该物种染色体动态和进化的认识。
