Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, USACH, Alameda 3363, Santiago, Chile.
Centro de Biotecnología Acuícola, Universidad de Santiago de Chile, USACH, Alameda 3363, Santiago, Chile.
Immunotherapy. 2021 Mar;13(4):309-326. doi: 10.2217/imt-2020-0209. Epub 2021 Jan 5.
Whole dead tumor cells can be used as antigen source and the induction of protective immune response could be enhanced by damage-associated molecular patterns. We generated whole dead tumor cells called B16-immunogenic cell bodies (ICBs) from B16 melanoma cells by nutrient starvation and evaluated the antitumor effect of B16-ICBs plus ATP and polymyxin B (PMB). The subcutaneous immunization with B16-ICBs + PMB + ATP a 50% of tumor-free animals and induced a significant delay in tumor growth in a prophylactic approach. These results correlated with maturation of bone marrow-derived dendritic cells and activation of T CD8 lymphocytes . Altogether, ICB + ATP + PMB is efficient in inducing the antitumor efficacy of the whole dead tumor cells vaccine.
全死肿瘤细胞可作为抗原来源，通过损伤相关分子模式可增强保护性免疫反应。我们通过营养饥饿从 B16 黑色素瘤细胞中产生了全死肿瘤细胞，称为 B16 免疫原性细胞体 (ICB)，并评估了 B16-ICB 加 ATP 和多粘菌素 B (PMB)的抗肿瘤作用。皮下免疫接种 B16-ICB + PMB + ATP 可使 50%的动物无肿瘤，并在预防性治疗中显著延迟肿瘤生长。这些结果与骨髓来源的树突状细胞的成熟和 T CD8 淋巴细胞的激活相关。总之，ICB + ATP + PMB 可有效诱导全死肿瘤细胞疫苗的抗肿瘤疗效。
