J Biomed Nanotechnol. 2020 Aug 1;16(8):1314-1323. doi: 10.1166/jbn.2020.2964.
Hemoglobin-polydopamine particles (Hb-PDA) have shown high stability, with polydopamine (PDA) serving as a protective layer and antioxidant. However, the effects of the PDA coating on the properties and biosafety of Hb-PDA remain unclear. This work was conducted to characterize Hb-PDA and evaluate its biosafety. Hb-PDA exhibited negative surface charge and their infusion did not cause blood immunotoxicity or significant tissue injury. Hb-PDA were not phagocyted after co-incubation with macrophages for 3 h. Moreover, the particles showed the highest accumulation in the lungs, and a prolonged retention in major organs. It was also found that the particles were cleared by macrophages in splenic tissue and Kupffer cells in hepatic tissue. In summary, this research showed that Hb-PDA has high dispersion stability, low toxicity, and extended retention, illustrating its potency as a biosafe oxygen carrier.
血红蛋白-聚多巴胺颗粒（Hb-PDA）具有高稳定性，聚多巴胺（PDA）作为保护层和抗氧化剂。然而，PDA 涂层对 Hb-PDA 的性质和生物安全性的影响尚不清楚。本研究旨在对 Hb-PDA 进行表征并评估其生物安全性。Hb-PDA 表现出负表面电荷，其输注不会引起血液免疫毒性或显著的组织损伤。Hb-PDA 在与巨噬细胞共孵育 3 小时后不会被吞噬。此外，这些颗粒在肺部的积累最高，在主要器官中的保留时间也较长。研究还发现，颗粒在脾组织中的巨噬细胞和肝组织中的枯否细胞中被清除。总之，这项研究表明，Hb-PDA 具有高分散稳定性、低毒性和延长的保留时间，表明其作为一种生物安全的氧载体具有潜力。
