Wu Naxin, Huang He, Min Taoling, Hu Haifeng
Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203, China.
School of Pharmacy, Fudan University, Shanghai 201203, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Dec 25;36(12):2685-2694. doi: 10.13345/j.cjb.200317.
Streptomyces aureofaciens DM-1 is a high-yielding 6-demethylchlortetracycline producer. The genome sequencing of DM-1 reveals a linear chromosome containing 6 824 334 bps nucleotides with GC content of 72.6%. In this genome, a total of 6 431 open reading frames were predicted by using glimmer 3.02, Genemark and Z-Curve softwares. Twenty-eight secondary metabolite biosynthetic gene clusters were uncovered by using AntiSMASH gene prediction software, including the complete 6-demethylchlortetracycline biosynthetic gene cluster. A frame-shift mutation in methyltransferase coding region was detected, which may result in the demethylation of chlortetracycline. The complete genome sequence of S. aureofaciens DM-1 provides basic information for functional genomics studies and selection of high-yielding strains for 6-demethylchlortetracycline.
金色链霉菌DM-1是6-去甲基金霉素的高产生产者。对DM-1进行的基因组测序显示，其线性染色体含有6824334个碱基对核苷酸，GC含量为72.6%。在该基因组中，使用glimmer 3.02、Genemark和Z曲线软件共预测出6431个开放阅读框。使用AntiSMASH基因预测软件发现了28个次生代谢物生物合成基因簇，包括完整的6-去甲基金霉素生物合成基因簇。检测到甲基转移酶编码区域的一个移码突变，这可能导致金霉素的去甲基化。金色链霉菌DM-1的完整基因组序列为功能基因组学研究以及6-去甲基金霉素高产菌株的选育提供了基础信息。
