Duan Chunyan, Zhang Kay, Duan Yizhong
Agricultural College, Henan University of Science and Technology, Luoyang 471003, Henan, China.
College of Life Sciences, Yulin University, Yulin 719000, Shaanxi, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Dec 25;36(12):2850-2859. doi: 10.13345/j.cjb.200188.
Amygdalus pedunculata Pall. is one of the 12 important woody oil crops in China. In this study, we determined the complete chloroplast genome sequence of A. pedunculata Pall. (MG602257) from Mu Us desert in Yulin city, Shaanxi province, China. The plastome was 157 851 bp with 36.8% GC content. Comparisons among the plastomes of MG602257 and other two MG869261 and KY101153 from Genebank of NCBI showed that the total length of these chloroplast genomes was MG602257< MG869261<KY101153, the GC contents of these genomes were same, and 149 mutation events including 93 substitutions and 56 indels were accurately located. The codon numbers and RSCU values among the three were similar. They shared the same genes at the four boundaries of the LSC, SSC, IRa, and IRb regions. The lengths of ndhF at the LSC/IRa boundary in MG602257 and MG869261 were same while adjusting 9 bp in KY101153. The length of ycf1 gene at the SSC/IRb boundary in MG602257, MG869261 and KY101153 was 5 657, 5 588 and 5 684 bp, respectively, which was the one of the reasons for the size difference among the three plastomes. Phylogenetic analyses based on 13 complete plastomes of Rosaceae species confirmed close relationships between A. trilob and A. pedunculata.
扁桃是中国12种重要的木本油料作物之一。在本研究中，我们测定了来自中国陕西省榆林市毛乌素沙漠的扁桃（MG602257）的完整叶绿体基因组序列。该质体基因组为157851 bp，GC含量为36.8%。将MG602257的质体基因组与NCBI基因库中的其他两个MG869261和KY101153进行比较，结果表明这些叶绿体基因组的总长度为MG602257 < MG869261 < KY101153，这些基因组的GC含量相同，并且准确定位了149个突变事件，包括93个替换和56个插入缺失。三者之间的密码子数量和RSCU值相似。它们在LSC、SSC、IRa和IRb区域的四个边界处具有相同的基因。MG602257和MG869261在LSC/IRa边界处的ndhF长度相同，而KY101153调整了9 bp。MG602257、MG869261和KY101153在SSC/IRb边界处的ycf1基因长度分别为5657、5588和5684 bp，这是三个质体基因组大小差异的原因之一。基于蔷薇科13个完整质体基因组的系统发育分析证实了扁桃和长柄扁桃之间的密切关系。
