Department of Electronic & Electrical Engineering, University College London, Gower St, Bloomsbury, London WC1E 6BT, UK.
Quadram Institute Biosciences, Norwich NR4 7UQ, UK.
IET Syst Biol. 2020 Dec;14(6):334-342. doi: 10.1049/iet-syb.2020.0007.
A simple model for the B-riboswitch regulatory network in is first described and the same analysis is applied when changing the strain to . Model validation is undertaken by linking the dynamics of the riboswitch model to bacterial growth and comparing the results obtained with in vivo experimental measurements. Measurements of bacterial growth are relatively straightforward to obtain experimentally, but experimental measurements relating to the operation of the riboswitch are more difficult. Using the validated model, sliding mode observer design methods are used to estimate given measurements of the concentration of vitamin B. The sliding mode approach is selected because of its inherent robustness properties as well as for the ease of implementation. Validation of the estimates of produced by the observer is undertaken by comparing the and vitamin B concentrations estimated from the observer with green fluorescent protein production and the concentration of vitamin B obtained experimentally. These experimental results also provide further validation of the underpinning mathematical model. The results establish that using a sliding mode observer as a soft sensor is a useful approach to explore the operation of a vitamin B riboswitch given measurements of the concentration of vitamin B.
首先描述了 中的 B-核糖开关调控网络的简单模型，然后当改变菌株为 时应用相同的分析。通过将核糖开关模型的动力学与细菌生长联系起来，并将获得的结果与体内实验测量进行比较，对模型进行了验证。细菌生长的测量在实验上相对容易获得，但与核糖开关的操作相关的实验测量则更为困难。使用经过验证的模型，采用滑模观测器设计方法来估计 ，其依据是测量维生素 B 的浓度。之所以选择滑模方法，是因为它具有固有的鲁棒性以及易于实现的特点。通过将观测器估计的 和维生素 B 浓度与绿色荧光蛋白产量和实验获得的维生素 B 浓度进行比较，对观测器产生的 的估计值进行了验证。这些实验结果还进一步验证了基础数学模型。结果表明，使用滑模观测器作为软传感器，在给定维生素 B 浓度测量的情况下，是一种探索维生素 B 核糖开关操作的有用方法。
