Key Laboratory of State Administration of Traditional Chinese Medicine for Production & Development of Cantonese Medicinal Materials/School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China.
Beijing Center for Physical and Chemical Analysis, Beijing, China.
Pharm Biol. 2021 Dec;59(1):40-46. doi: 10.1080/13880209.2020.1865409.
The traditional drying method, sun drying, for Ramat. cv. Hang-ju (Compositae) (HJ) is widely replaced by sulphur fumigation (SF), which has an unknown effect on its efficacy.
To investigate protective effects of nonfumigated HJ (NHJ) and sulphur-fumigated HJ (SHJ) water extracts against oxidative stress and lipid peroxidation.
Sprague-Dawley rats were administered high-fat diet to induce hyperlipidaemia and randomly divided into eight groups ( = 6): control, fenofibrate, NHJ and SHJ extracts (1, 2 or 4 g crude drugs/kg/d; intragastric administration for 8 weeks). Serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), superoxide dismutase (SOD) and malondialdehyde (MDA) levels were detected. Human umbilical vein endothelial cells (HUVECs) were treated with NHJ and SHJ extracts (50, 100 or 200 μg/mL) for 24 h, followed by oxidized low-density lipoprotein (ox-LDL, 20 μg/mL) for 2 h . Cellular reactive oxygen species (ROS), SOD and MDA levels and apoptosis were evaluated.
NHJ was more effective than SHJ in decreasing serum TG, TC, LDL-C, LDL/HDL and MDA while increasing serum HDL-C and SOD levels at high doses. SHJ (IC=19.9 mg/mL) suppressed HUVEC growth stronger than NHJ (IC=186.7 mg/mL). At 200 μg/mL, NHJ was more effective than SHJ in downregulating ROS and MDA levels, reducing HUVECs apoptosis rate and elevating SOD activity in ox-LDL-treated HUVECs.
SF causes oxidative damage and attenuates antioxidative activity in ox-LDL-treated HUVECs, which promotes lipid peroxidation. SF is not recommended for processing HJ.
传统的干燥方法——日晒，已广泛被硫磺熏蒸（SF）所取代，用于对杭菊（菊科）（HJ）进行干燥，但这种替代方法对其功效的影响尚不清楚。
研究未经熏硫的杭菊（NHJ）和硫磺熏制的杭菊（SHJ）水提取物对氧化应激和脂质过氧化的保护作用。
将 Sprague-Dawley 大鼠给予高脂饮食以诱导高脂血症，并将其随机分为八组（每组 6 只）：对照组、非诺贝特组、NHJ 和 SHJ 提取物（1、2 或 4 g 生药/kg/d；灌胃 8 周）。检测血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）、超氧化物歧化酶（SOD）和丙二醛（MDA）水平。将人脐静脉内皮细胞（HUVECs）用 NHJ 和 SHJ 提取物（50、100 或 200μg/mL）处理 24 h，然后用氧化型低密度脂蛋白（ox-LDL，20μg/mL）处理 2 h。评估细胞内活性氧（ROS）、SOD 和 MDA 水平及细胞凋亡。
NHJ 比 SHJ 更能有效降低血清 TG、TC、LDL-C、LDL/HDL 和 MDA 水平，同时增加血清 HDL-C 和 SOD 水平（高剂量时）。SHJ（IC=19.9μg/mL）对 HUVEC 生长的抑制作用强于 NHJ（IC=186.7μg/mL）。在 200μg/mL 时，NHJ 比 SHJ 更能有效降低 ROS 和 MDA 水平，降低 ox-LDL 处理的 HUVEC 细胞凋亡率，并提高 SOD 活性。
SF 会导致 ox-LDL 处理的 HUVEC 氧化损伤和抗氧化活性减弱，从而促进脂质过氧化。SF 不建议用于 HJ 的加工。
