Laboratorio de Virología, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad de Belgrano, Villanueva 1324, C1426BMJ, Buenos Aires, Argentina.
Virus Genes. 2021 Feb;57(1):106-110. doi: 10.1007/s11262-020-01816-7. Epub 2021 Jan 5.
Asian macaques infected with simian immunodeficiency viruses (SIVs) isolated from African non-human primates develop a disease similar to human AIDS. SIV enters its target cells by binding to CD4 and a coreceptor, typically CCR5. Maraviroc is an entry inhibitor of human immunodeficiency virus type 1 (HIV-1) that prevents the interaction between CCR5 and the surface subunit gp120 of the viral envelope glycoprotein (Env). Thus far, the activity of maraviroc on SIV entry has been poorly studied. Here, we determined in vitro pharmacological parameters of the effect of maraviroc on the SIV Env association with CCR5. Cell-to-cell fusion inhibition assays were used to compare the susceptibility to maraviroc of the SIVsmmPBj Env-CCR5 interaction with that of HIV-1BaL Env. Analysis of dose-response curves and determination of IC values demonstrate that increasing concentrations of maraviroc inhibit the membrane fusion activity of SIVsmmPBj Env in a manner and to an extent similar to that of HIV-1BaL Env.
感染源自非洲非人灵长类动物的猴免疫缺陷病毒（SIV）的亚洲猕猴会发展出类似于人类艾滋病的疾病。SIV 通过与 CD4 和辅助受体（通常是 CCR5）结合进入其靶细胞。马拉维若（maraviroc）是一种人类免疫缺陷病毒 1（HIV-1）进入抑制剂，可阻止 CCR5 与病毒包膜糖蛋白（Env）表面亚基 gp120 之间的相互作用。到目前为止，对马拉维若（maraviroc）对 SIV 进入的作用的体外药理学参数的研究还很少。在这里，我们确定了马拉维若（maraviroc）对 SIV Env 与 CCR5 相互作用的体外药理学参数。细胞间融合抑制测定用于比较 SIVsmmPBj Env-CCR5 相互作用对马拉维若（maraviroc）的敏感性与 HIV-1BaL Env 的敏感性。剂量反应曲线分析和 IC 值的确定表明，马拉维若（maraviroc）的浓度增加以与 HIV-1BaL Env 相似的方式和程度抑制 SIVsmmPBj Env 的膜融合活性。
